Chinese Journal OF Rice Science >
Effects of OsESV1 on Starch Synthesis in Rice
Received date: 2021-03-19
Revised date: 2021-05-12
Online published: 2022-03-11
【Objective】 It is important to reveal the role of EARLY STARVATION1 (OsESV1) in starch metabolism of rice.【Method】 The osesv1 mutants were obtained by using the CRISPR-Cas9 system. The phenotype and the physicochemical properties of endosperm starch of osesv1 were investigated. The expression pattern and function of OsESV1 were analyzed. 【Result】 The OsESV1 protein was conserved in plants. Compared with the wild type, the osesv1 mutants exhibited lower plant height, panicle length, and grain number per panicle, but higher tiller number. Though the starch contents in osesv1 leaves significantly decreased, the total starch contents of osesv1 grains did not obviously altered and the amylose contents increased. The expression of OsESV1 was constitutive and rhythmic. OsESV1 was located in chloroplasts with a dot-like distribution. Yeast two-hybrid and bimolecular fluorescence complementation(BiFC) assays showed that OsESV1 could interact with ADP glucose pyrophosphorylase small subunit (OsAGPS) 2a and OsAGPS1.【Conclusion】 OsESV1 significantly affects the starch synthesis pathway in rice leaves, while not in endosperm.
Key words： rice; starch; endosperm; OsESV1; interaction
WANG Yongxiang, YAN Hangang, XU Hancong, FU Yushuang, SHAN Zhuangzhuang, HU Xiaoqing, ZHANG Wenwei, JIANG Ling . Effects of OsESV1 on Starch Synthesis in Rice[J]. Chinese Journal OF Rice Science, 2022 , 36(2) : 139 -149 . DOI: 10.16819/j.1001-7216.2022.210308
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