Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2015 , Vol. 29 >Issue 4: 305 - 310

DOI: https://doi.org/10．3969/j．issn．1001G7216．2015．03．010

ResearchPaper

Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/z-t

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  • 1 The Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
    2.Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China
# These authors contributed equally to this work; *Corresponding author, E-mail: bsujing@126.com

Received date: 2014-05-20

  Revised date: 2014-07-15

  Online published: 2015-07-10

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Abstract

Specific molecular markers for detecting the alleles of Pi2/Pi9/Piz-t, designated as Pi2SNP, Pi9SNP and Pizt-PA, respectively, have been successfully developed through sequence comparison method. These three specific markers can distinctly distinguish target genes from others mapped on Pi2/9 locus, which facilitate the molecular marker-assistant selection and the development of pyramiding cultivars combined with other allele R genes. Additionally, 101 main cultivars and breeding parents collected from different rice-planting areas have been diagnosed with these three specific markers, and the results showed that only 2 out of 101 cultivars carried Piz-t, but neither Pi2 nor Pi9 band had been detected. So far, it indicated that most of the main cultivars and breeding parents in China do not carry Pi2/Pi9/Piz-t. These results had provided important information for the application of Pi2/Pi9/Piz-t in the blast resistance breeding programs.

Key words： rice; blast; resistance gene; molecular marker; MAS; Pi2/9 locus

Cite this article

Li-xia HUA, Wen-juan WANG, Shen CHEN, Cong-ying WANG, Lie-xian ZENG, Jian-yuan YANG, Xiao-yuan ZHU, Jing SU . Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/z-t[J]. Chinese Journal OF Rice Science, 2015 , 29(4) : 305 -310 . DOI: 10．3969/j．issn．1001G7216．2015．03．010

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