Chinese Journal OF Rice Science >
Comprehensive Evaluation and QTL Analysis for Flag Leaf Traits Using a Backcross Population in Rice
Received date: 2021-01-12
Revised date: 2021-03-20
Online published: 2021-11-10
【Objective】In order to clarify the correlations between flag leaf-related traits and their relationships with six agronomic traits, a comprehensive evaluation of flag leaf traits was made. The identification of QTLs associated with flag leaf traits will lay a foundation for fine mapping and cloning of the functional genes underlying rice flag leaf-related traits, which is helpful to breed elite cultivars with excellent flag leaf traits. 【Method】A backcross population with two generations derived from an elite japonica rice cultivar Koshihikari (recipient) and a Portuguese rice landrace Bertone (donor) was used as plant materials. The genetic linkage maps were constructed by using SSR markers with the BC3F1 population. Several parameters including flag leaf SPAD, flag leaf length, flag leaf width, length to width ratio of flag leaf as well as flag leaf area were measured in the parental varieties and BC3F2 population. In order to analyze the relationship between D value and the six agronomic traits, membership functions and the index weight method were used to comprehensively evaluate the flag leaf traits of these 260 BC3F2 lines. Single-point analysis (SPA) and interval mapping (IM) were used to identify QTLs for traits related to flag leaf in rice.【Result】After the flag leaf was fully expanded, the flag leaf SPAD values of the two parents showed a dynamic change, increasing at first and reduced subsequently. The five flag leaf-related traits of the BC3F2 population exhibited diverse phenotypic variations, whose overall performance resembled the recurrent parent (Koshihikari). The results revealed that four morphological traits of flag leaf were significantly correlated with each other, while there were no significant correlations with flag leaf SPAD. Using principal component analysis (PCA) and stepwise regression analysis, flag leaf width, flag leaf SPAD, flag leaf length and flag leaf area were selected as the key indexes of D value out of the flag leaf comprehensive evaluation. The results showed that except for tiller number and effective panicle number, the other four traits including plant height, panicle length, basal culm thickness and grain yield per plant were all significantly higher in lines with a higher D value than that in lines with a small D value. A total of 18 QTLs were detected, which were located on chromosomes 1, 4, 7 and 8 with phenotypic variation explained ranging from 4.00% to 28.00% (SPA) and 3.41% to 27.00% (IM), respectively. Except for qFLSPAD1, the other 17 QTLs showed synergistic effect from Bertone alleles. Moreover, a QTL cluster was found in the RM22720–RM404 interval on chromosome 8, containing six major QTLs(qFLL8.1, qFLL8.2, qFLA8.1, qFLA8.2, qD8.1 and qD8.2). 【Conclusion】Flag leaf width, flag leaf SPAD, flag leaf length and flag leaf area were the important indicators that affect the comprehensive evaluation of flag leaf. The flag leaf traits were significantly or extremely significantly positively correlated with the yield per plant. Here we identified 18 QTLs for flag leaf traits and a QTL cluster detected in the RM22720–RM404 interval on chromosome 8, which is an important chromosomal region affecting flag leaf-related traits.
Key words： rice; backcross population; flag leaf traits; comprehensive evaluation; QTL mapping
Jie LI, Rongrong TIAN, Tianliang BAI, Chunyan ZHU, Jiawei SONG, Lei TIAN, Shuaiguo MA, Jiandong LÜ, Hui HU, Zhenyu WANG, Chengke LUO, Yinxia ZHANG, Peifu LI . Comprehensive Evaluation and QTL Analysis for Flag Leaf Traits Using a Backcross Population in Rice[J]. Chinese Journal OF Rice Science, 2021 , 35(6) : 573 -585 . DOI: 10.16819/j.1001-7216.2021.210110
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