Chinese Journal OF Rice Science >
Response Characteristics of Rice Cysteine-rich Receptor-like Kinases Family Genes to Rhizoctonia solani and Plant Hormones
Received date: 2021-03-06
Revised date: 2021-03-25
Online published: 2021-09-10
【Objective】 Clarifying the response characteristics of rice cysteine-rich receptor-like kinases (CRKs) family genes to Rhizoctonia solani and plant hormones is important preliminary work to analyze the functions of CRKs in rice resistance to R. solani. 【Method】 A phylogenetic tree of 45 CRKs in rice was constructed by bioinformatics. qPCR was used to analyze their response characteristics to R. solani, plant hormones ethylene (ET), jasmonic acid (JA), salicylic acid (SA) and cytokinin (CK), and their expression patterns in different tissues.【Result】 The rice CRKs family can be divided into four groups. Most of the CRKs clustered or closely distributed on chromosomes from the same group or branch. Forty-one CRKs responded to R. solani infection, and 17 of them responded strongly. Combined with their tissue expression patterns, it was found that 10 of the 17 CRKs, including CRK15, CRK23, CRK24, CRK26, CRK27, CRK28, CRK29, CRK30, CRK31 and CRK33, were expressed strongly in leaf sheathes and leaves, suggesting that these genes may be involved in regulating resistance to sheath blight. Most of CRKs from the same branch had similar response characteristics to R. solani, indicating that these CRKs may have functional redundancy in regulating resistance to R. solani. Forty CRKs responded to three or four kinds of plant hormones, and their responses to different hormones varied, indicating that CRKs might be widely involved in these plant hormones-mediated defense pathways. There were 17 CRKs with opposite responses to JA and SA, 21 with similar responses to JA and SA, 21 with similar responses to ET and JA, and 23 with similar responses to ET and SA, respectively. These genes may be involved in the interactions among ET, JA and SA. The results not only reflected the synergistic and antagonistic effects of ET, JA and SA signaling pathways, but also indicated that these genes may participate in the interactions among ET, JA and SA.【Conclusion】 Some rice CRKs involved in the regulation of rice sheath blight resistance have been identified, and they may play roles in the plant hormones-mediated defense pathways, which provides scientific clues for us to further explore the function of CRKs in regulating rice sheath blight resistance.
Key words： rice; OsCRK gene; sheath blight; defense-related hormone; response characteristics
Zhiming FENG, Guangda WANG, Jianhua ZHAO, Ran JU, Mengchen LI, Peng GAO, Keming HU, Zongxiang CHEN, Shimin ZUO . Response Characteristics of Rice Cysteine-rich Receptor-like Kinases Family Genes to Rhizoctonia solani and Plant Hormones[J]. Chinese Journal OF Rice Science, 2021 , 35(5) : 439 -448 . DOI: 10.16819/j.1001-7216.2021.210305
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