Chinese Journal OF Rice Science >
A New Approach for Fine-mapping and Map-based Cloning of Minor-Effect QTL in Rice
Received date: 2020-12-09
Revised date: 2021-01-24
Online published: 2021-07-10
Important agronomic traits in rice are generally controlled by a few major-effect QTLs and a large number of minor-effect QTLs. Great progresses have been made in the cloning of major QTLs, while minor QTLs remain difficult to be cloned due to their small genetic effects and the influence of measurement error. A new approach for fine-mapping and map-based cloning of rice minor-effect QTL was introduced in this article. The approach includes two steps: 1) Use the residual heterozygote to construct near isogenic lines for fine-mapping of the target QTL; 2) Use the genome editing to create mutants of candidate genes for gene function identification. Using the strategy, we fine-mapped six minor QTLs for grain weight and grain size on the long arm of chromosome 1, and successfully cloned the first minor QTL for grain weight. We expect that this approach could provide more options for QTL cloning and new germplasm creation.
Key words： rice (Oryza sativa L.); grain size; minor QTL; map-based cloning
Yujun ZHU, Ziwei ZUO, Zhenhua ZHANG, Yeyang FAN . A New Approach for Fine-mapping and Map-based Cloning of Minor-Effect QTL in Rice[J]. Chinese Journal OF Rice Science, 2021 , 35(4) : 407 -414 . DOI: 10.16819/j.1001-7216.2021.201206
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