Chinese Journal OF Rice Science >
Identification and Gene Mapping of a Lesion Mimic Mutant lm8015-2 in Rice
Received date: 2020-09-11
Revised date: 2020-11-13
Online published: 2021-07-10
【Objective】 To substantiate the genetic mechanisms behind the disease resistance of rice, we identified a rice lesion mimic mutant lm8015-2. 【Method】 lm8015-2 was obtained from an EMS-induced Zhonghui 8015 (ZH8015) mutant library. The SOD, CAT, POD activities, H2O2, MDA, SP, photosynthetic pigments contents, the expression level of defense genes were analyzed in lm8015-2 and its wild-type leaves. The population derived from 02428/ lm8015-2 was used for genetic analysis and fine mapping. 【Result】 Its red rust-like spots were first observed on the tips of the mutant leaves three weeks after sowing, then spread gradually to the whole leaf and throughout the plant at tillering stage, causing the death of whole leaf at heading stage. The shading assay showed that the lesions on the leaves of lm8015-2 were induced by natural light. Meanwhile, photosynthetic pigment contents of lm8015-2 decreased significantly. The staining experiments of EB and DAB showed the excessive H2O2 deposition and cell death of lm8015-2. Compared with the wild type, the activities of SOD and POD, the MDA content increased, while the content of SP decreased in the mutant lm8015-2. Genetic analysis suggested that the phenotype of lm8015-2 was controlled by a single nuclear recessive gene, which was located in a 104 kb interval between the markers W32-85 and C32-8 on chromosome 5. Sequencing analysis revealed that a single base substitution (T to A) of the initiation codon (ATG) occurred in the DNA sequence of LOC_Os05g48390, resulting in the absence of initiation codon. The qRT-PCR results showed the expression of partial defense genes were up-regulated obviously in lm8015-2. 【Conclusion】 The results reveal the target gene is allelic to LTN1, and the mutation of LM8015-2 likely enhances the expression of partial defense genes.
Key words： rice; lesion mimic mutant; fine mapping
Chen WANG, Beifang WANG, Yingxin ZHANG, Yongrun CAO, Yue ZHANG, Min JIANG, Kangji BIAN, Xiaohui ZHANG, Qunen LIU . Identification and Gene Mapping of a Lesion Mimic Mutant lm8015-2 in Rice[J]. Chinese Journal OF Rice Science, 2021 , 35(4) : 352 -358 . DOI: 10.16819/j.1001-7216.2021.200908
| [1] | Roohollah S D, Angelica L, Aliakbari M, Hadi P A.Plausible association between drought stress tolerance of barley (Hordeum vulgare L.) and programmed cell death via MC1 and TSN1 genes[J]. Physiologia Plantarum, 2020, 170(1): 46-59. |
| [2] | Lin C H, Pan Y C, Ye N H, Shih Y T, Liu F W, Chen C Y.LsGRP1, a class II glycine-rich protein of Lilium, confers plant resistance via mediating innate immune activation and inducing fungal programmed cell death[J]. Molecular Plant Pathology, 2020, 21(9): 1149-1166. |
| [3] | Niazian M, Shariatpanahi M E.In vitro-based doubled haploid production: Recent improvements[J]. Euphytica, 2020, 216(5): 1-21. |
| [4] | 张宏根, 王茂宇, 张丽佳, 胡雅, 马佳琦, 张翼帆, 汤述翥, 梁国华, 顾铭洪. 水稻类病斑突变体wy3的鉴定和基因定位[J]. 中国水稻科学, 2016, 30(3): 239-246. |
| [4] | Zhang H G, Wang M Y, Zhang L J, Hu Y, Ma J Q, Zhang Y F, Tang S Z, Liang G H, Gu M H.Characterization and gene mapping of lesion mimic mutant wy3 in rice[J]. Chinese Journal of Rice Science, 2016, 30(3): 239-246. (in Chinese with English abstract) |
| [5] | Shirasu K, Schulze-Lefert P.Regulators of cell death in disease resistance[J]. Plant Molecular Biology, 2000, 44(3): 371-385. |
| [6] | Yuan Y, Zhong S, Li Q, Zhu Z, Lou Y, Wang L, Wang J, Wang M, Yang D, Li Q, He Z.Functional analysis of rice NPR1-like genes reveals that OsNPR1/NH1 is the rice orthologue conferring disease resistance with enhanced herbivore susceptibility[J]. Plant Biotechnology Journal, 2010, 5(2): 313-324. |
| [7] | Yamanouchi U, Yano M, Lin H X, Ashikari M, Yamada K.A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein[J]. Proceedings of the National Academy of Sciences, 2002, 99(11): 7530-7535. |
| [8] | You Q Y, Zhai K, Yang D L, Yang W B, Wu J N, Liu J Z, Pan W B, Wang J J, Zhu X D, Jian Y K, Liu J Y, Zhang Y Y, Li Q, Lou Y G, Xie Q, He Z H.An E3 ubiquitin ligase-BAG protein module controls plant innate immunity and broad-spectrum disease resistance[J]. Cell Host＆Microbe, 2016, 20(6): 758-769. |
| [9] | Fekih R, Tamiru M, Kanzaki H, Abe A, Yoshida K, Kanzaki E, Saitoh H, Takagi H, Natsume S, Undan J R, Undan J, Terauchi R.The rice (Oryza sativa L.) LESION MIMIC RESEMBLING, which encodes an AAA-type ATPase, is implicated in defense response[J]. Molecular Genetics & Genomics, 2015, 290(2): 611-622. |
| [10] | Arnon D I.Copper enzyme in isolated chloroplasts: Polyphenoloxidase in Beta vulgaris[J]. Plant Physiology, 1949, 24(1): 1-15. |
| [11] | Wellburn A R.The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution[J]. Journal of Plant Physiology, 1994, 144(3): 307-313. |
| [12] | Thordal-Christensen H, Zhang Z G, Wei Y D, Collinge D B.Subcellular localization of H2O2 in plants: H2O2 accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction[J]. Plant Journal, 1997, 11(6): 1187-1194. |
| [13] | Li R, L S Y, Xu Z X. Studies on the programmed cell death in rice during starchy endosperm development[J]. Agricultural Sciences in China, 2004, 3(9): 663-670. |
| [14] | Rogers S O, Bendich A J.Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues[J]. Plant Molecular Biology, 1985, 5(2): 69-76. |
| [15] | 孙德权, 郭启高, 胡玉林, 谢江辉. 改良Trizol法提取香蕉叶片总RNA[J]. 广东农业科学, 2009(5): 162-164. |
| [15] | Sun D Q, Guo Q G, Hu Y L, Xie J H.Extraction of total RNA from banana with improved Trizol method[J]. Guangdong Agricultural Sciences, 2009(5): 162-164. (in Chinese) |
| [16] | Cao Y, Yan Y, Zhang F, Wang H D, Gu M, Wu X N, Sun S B, Xu G H.Fine characterization of OsPHO2 knockout mutants reveals its key role in Pi utilization in rice[J]. Journal of Plant Physiology, 2014, 171(3-4): 340-348. |
| [17] | Pickart C M, Eddins M J.Ubiquitin: Structures, functions, mechanisms[J]. Biochimica et Biophysica Acta, 2004, 1695(1-3): 55-72. |
| [18] | Zeng L R, Qu S H, Bordeos A, Yang C W, Baraoidan M, Yan H Y, Xie Q, Nahm B H, Leung H, Wang G L.Spotted leaf11, a negative regulator of plant cell death and defense, encodes a U-box/armadillo repeat protein endowed with E3 ubiquitin ligase activity[J]. The Plant Cell, 2004, 16(10): 2795-2808. |
| [19] | Ma J, Wang Y F, Ma X D, Meng L Z, Jing R N, Wang F, Wang S, Cheng Z J, Zhang X, Jiang L, Wang J L, Wang J, Zhao Z C, Zhao Z C, Guo X P, Lin Q B, Wu F Q, Zhu S S, Wu C Y, Ren Y L, Lei C, Zhai H Q, Wan J M.Disruption of gene SPL35, encoding a novel CUE domain-containing protein, leads to cell death and enhanced disease response in rice[J]. Plant Biotechnology Journal, 2019, 17(8): 1679-1693. |
| [20] | Hu B, Zhu C, Li F, Tang J Y, Wang Y Q, Lin A H, Liu L C, Che R H, Chu C C.LEAF TIP NECROSIS1 plays a pivotal role in the regulation of multiple phosphate starvation responses in rice[J]. Plant Physiology, 2011, 156(3): 1101-1115. |
| [21] | Hu B, Wang W, Deng K, Li H, Zhang Z H, Zhang L H, Chu C C.MicroRNA399 is involved in multiple nutrient starvation responses in rice[J]. Frontiers in Plant Science, 2015, 188(6): 1-10. |
| [22] | E Z G, Zhang Y P, Li T T, Wang L, Zhao H M. Characterization of the ubiquitin-conjugating enzyme gene family in rice and evaluation of expression profiles under abiotic stresses and hormone treatments[J]. PloS One, 2015, 10(4): e0122621. |
| [23] | 乐美旺, 陈实, 潘庆华, 曾任森, 刘迎湖, 骆世明. 水稻和稻瘟病菌互作中的信号传导及防御反应基因诱导表达的研究[J]. 植物病理学报, 2007, 37(1): 42-49. |
| [23] | Le M W, Chen S, Pan Q H, Zeng R S, Liu Y H, Tuo S M.Induced expression of signaling pathway and defense-related genes in interaction between rice and Magnaporthe grisea[J]. Acta Phytopathologica Sinica, 2007, 37(1): 42-49. (in Chinese with English abstract) |
/
| 〈 |
|
〉 |