Chinese Journal OF Rice Science >
Changes in Endogenous Hormones in Rice Seedlings Infested by White-backed Planthoppers
#These authors contributed equally to this work
Received date: 2020-08-22
Revised date: 2020-09-16
Online published: 2021-03-10
【Objective】It is of great importance to study the changes of endogenous hormone concentration and relative expression level of associated genes in rice seedling after infestation by white-backed planthoppers (WBPH), which would provide reference for insight into WBPH resistance mechanism regulated by endogenous hormones. 【Method】Salicylic acid (SA), abscisic acid (ABA), jasmonic acid (JA), and auxin (IAA) in susceptible line 9311 and near isogenic line (NIL) of resistance were quantified using UPLC-MS at 0, 24, and 48 h after infestation by WBPH; and the relative expression levels of five associated genes between resistant and susceptible lines were detected by qRT-PCR. 【Result】SA concentration in NIL increased at 24 h after WBPH infestation and decreased at 48 h after WBPH infestation as compared to the untreated line; while it was on the contrary in 9311. ABA concentration was relatively stable in NIL after WBPH infestation, but increased at 24 h and then decreased at 48 h after WBPH infestation in 9311. Both IAA and JA concentration continuously increased in resistant and susceptible lines after infestation treatment. The result of qRT-PCR indicated that the relative expression levels of hormone pathway-related genes OsPAL06, OsZEP, OsLOX, and OsYUCCA1 were significantly different at 24 h after WBPH infestation between resistant and susceptible lines, but no significant difference was observed at 0 and 48 h after WBPH infestation. However, the relative expression level of OsICS1 showed significant difference at 48 h after WBPH infestation between the resistant and susceptible lines; whereas no significant difference was detected at 0 or 24 h after WBPH infestation. 【Conclusion】JA and ABA concentration in the resistant plants were higher than those in the susceptible ones, which was possibly associated with the resistant genes. SA concentration and the relative expression level of its associated genes OsPAL06 and OsICS1 indicated that the resistant line responded more quickly to WBPH infestation, which suggested that it might play an important role during insect infestation.
Key words： rice; Sogatella furcifera (Horváth); endogenous hormones; qRT-PCR
Xuelu WEI, Baiyi LU, Fugang HUANG, Fahuo LI, Fang LIU, Yongfu QIU . Changes in Endogenous Hormones in Rice Seedlings Infested by White-backed Planthoppers[J]. Chinese Journal OF Rice Science, 2021 , 35(2) : 200 -206 . DOI: 10.16819/j.1001-7216.2021.0815
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