Chinese Journal OF Rice Science >
Identification and Genetic Analysis of Cross-incompatibility ina RiceDW-type Sterile Line
Received date: 2020-06-30
Revised date: 2020-09-29
Online published: 2020-11-10
【Objective】In the process of breeding DW-type three-line hybrid rice, cross incompatibility (CI) was found when the cytoplasmic male sterile (CMS) lines were crossed/backcrossed with some varieties. We focused on the traittolayatheoretical basis for improving CI of DW-type CMS lines, so as to promote the application ofDW-type three-line. 【Method】With isocytoplasmallonuclear materials of DW-type and isonuclear alloplasmic male sterile lines of WA-type as female parents, maintainer lines as male parents, cross experimentswere carried out to investigate the cause of CI. Genetic analysis and gene rough mapping were conducted using a set of chromosome single segment substitution lines, which were crossed to a DW-type CMS line DY1A, respectively.【Result】The CI was caused by nuclear-cytoplasmic interactions. Two types of CI were observed that the first type occurred in F0 crosses and another after backcrossing. For the first type, primarily genetic analysis showed that the CI was controlled by a recessive gene in the nucleus, temporarily named CI1(t), which was then mapped between the two markers RM6838 and RM5767, linked toRM3395,on chromosome 8.【Conclusion】The CI of DW-type CMS linescould beimprovedby molecular methods.
Tao XIONG, Yuanyuan NIE, Fangming MAO, Jianguo LEI, Linghua MAO, Shan ZHU, Renliang HUANG, Xianhua SHEN, Song YAN . Identification and Genetic Analysis of Cross-incompatibility ina RiceDW-type Sterile Line[J]. Chinese Journal OF Rice Science, 2020 , 34(6) : 520 -524 . DOI: 10.16819/j.1001-7216.2020.0610
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