Chinese Journal OF Rice Science >
A Preliminary Study on the Function of Rice Heading Date Gene OsDof6
Received date: 2020-02-20
Revised date: 2020-05-19
Online published: 2020-09-10
【Objective】Our goal is to further explore the expression pattern and biological function of OsDof6, a rice Dof family transcription factor screened with the panicle development chip in the previous study. 【Method】Bioinformatics approaches were applied to analyze OsDof6 gene, protein and protein promoter sequences. The gene was edited using CRISPR/Cas9 technology. Real-time quantitative PCR and subcellular localization technology was used to reveal the gene expression pattern. 【Result】The gene promoter contains a large number of photo-, hormone-, and stress-responses and tissue expression elements. Then, two mutants of different mutation types 9522Dof6-3 and 9522Dof6-4 were obtained by using CRISPR/Cas9 technology. Through observation of the T1 generation of the mutant materials, we found that the mutants had significantly less tillers and longer heading dates (delayed by 3 days) compared to the control materials. Real-time quantitative PCR results showed that OsDof6 was expressed in varying degrees in the roots, stems, leaves and panicles, and the relative expression was significantly increased at late stages of panicle development. Subcellular localization analysis showed that OsDof6 belongs to a nuclear localization gene. 【Conclusion】OsDof6 gene affects the tiller number and heading date of rice.
Key words： rice; Dof family; heading date; CRISPR/Cas9
Licheng ZHANG, Yixing LI, Tiankang WANG, Mudan QIU, Shufeng SONG, Hao DONG, Lei LI, Jianfeng LIU, Li LI . A Preliminary Study on the Function of Rice Heading Date Gene OsDof6[J]. Chinese Journal OF Rice Science, 2020 , 34(5) : 397 -405 . DOI: 10.16819/j.1001-7216.2020.0211
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