Chinese Journal OF Rice Science >
Detection and Analysis of Magnaporthe oryzae Avirulent Gene AVR-Pita and Its Homologous Genes in Heilongjiang Province
Received date: 2019-07-21
Revised date: 2019-11-21
Online published: 2020-03-10
【Objective】We aim to investigate the distribution and variation of avirulent AVR-Pita family of Magnaporthe oryzae from Heilongjiang Province, and to understand the pathogenic phenotypes of avirulence alleles.【Methods】Specific primers of the three avirulent genes AVR-Pita1, AVR-Pita2 and AVR-Pita3 were used. 202 M. oryzae single-spore isolates were obtained from different regions of Heilongjiang Province, their DNA were PCR-amplified by using avirulent genes primers in 2017. PCR products of representative strains falling into different types and from different aresa were selected and sequenced by using agarose gel electrophoresis detection. The base and amino acid sequences were compared with those of the corresponding avirulent genes, and the rice resistant single gene line was used to verify the function of different strains of Magnaporthe oryzae.【Results】The frequency of AVR-Pita1 is 36.14%, and that of AVR-Pita3 is 59.41%. The target band of AVR-Pita2 could not be amplified from the DNA of 202 strains from Heilongjiang Province. Sequence analysis of some PCR products of AVR-Pita1 and AVR-Pita3 were carried out. Five variation types of AVR-Pita1 were detected; they were AVR-Pita1-1, AVR-Pita1-A, AVR-Pita1-B, AVR-Pita1-C and AVR-Pita1-D. Functional verification showed that AVR-Pita1-1, AVR-Pita1-A, AVR-Pita1-B and AVR-Pita1-D lost their function. No mutant strain was detected in the virulent gene AVR-Pita3. 【Conclusion】 AVR-Pita1 had strong mutation ability, which led to the loss of avirulent function of most strains, this should be used in conjunction with other resistant genes. AVR-Pita2 was not found in Heilongjiang Province. The sequence of AVR-Pita3 gene was stable in the strain.
Feng MENG, Yaling ZHANG, Xuehui JIN . Detection and Analysis of Magnaporthe oryzae Avirulent Gene AVR-Pita and Its Homologous Genes in Heilongjiang Province[J]. Chinese Journal OF Rice Science, 2020 , 34(2) : 143 -149 . DOI: 10.16819/j.1001-7216.2020.9085
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