Chinese Journal OF Rice Science >
Expression Profiles and Functions of Small Heat Shock Proteins in Nilaparvata lugens
About author:#These authors contributed equally to this work
Received date: 2019-03-27
Revised date: 2019-06-14
Online published: 2020-01-10
【Objective】To explore the adaptation mechanisms of brown planthoppers(BPH) to temperature stress, the expression profiles and functions of small heat shock proteins (sHSPs) in Nilaparvata lugens was studied. 【Method】The nucleotide sequence of small heat shock proteins in N. lugens was screened from transcriptome database by BLAST; The sequence analysis was carried out by molecular biological software such as Bioedit and Mega; the expression profiles were analyzed by qPCR; and the prokaryotic expression technology was used for functional research. 【Result】Six sHsps (NlHsp20.9, NlHsp21.6, NlHsp21.9, NlHsp22.4, NlHsp23.1 and NlHsp28.7) with conserved alpha crystal structure were screened. The ORF length of these genes was 561, 531, 570, 570, 588 and 735 bp, and the theoretical isoelectric point was 5.96, 5.77, 6.32, 5.01, 5.74 and 7.74, respectively. The peak expression of NlHsp28.7 was found in the third larvae and the peak of NlHsp21.9 and NlHsp23.1 in females. The expression of these genes decreased in females after exposure to low temperature, while increased after exposure to high temperature except NlHsp22.4. To the 3rd instar nymphs, the expression of about half of sHsps decreased after low temperature stress and half of sHsps increased, while all increased after high temperature stress. Moreover, the survival of transformed BL21 (DE3) was significantly increased after heat treatment. 【Conclusion】The small heat shock protein of N. lugens has stage-specific and inducible expression characteristics and show protection against heat stress. It may play an important role in response to high temperature stress, but its role in response to low temperature stress is related to insect developmental stages.
Key words： Nilaparvata lugens; small heat shock proteins; temperature; expression
Lei PAN, Lihua WANG, Feng ZHU, Yangchun HAN, Pei WANG, Jichao FANG . Expression Profiles and Functions of Small Heat Shock Proteins in Nilaparvata lugens[J]. Chinese Journal OF Rice Science, 2020 , 34(1) : 37 -45 . DOI: 10.16819/j.1001-7216.2020.9034
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