Chinese Journal OF Rice Science >
Development and Verification of Specific Molecular Markers for Pigm Gene Associated with Broad-spectrum Resistance to Rice Blast
Received date: 2019-07-12
Revised date: 2019-11-20
Online published: 2020-01-10
【Objective】 Pigm from an indica rice variety Gumei 4, is one of the most important genes with broad-spectrum and durable resistance to rice blast. It is very necessary to improve its selection efficiency by developing specific and co-dominant markers in breeding. 【Method】The molecular markers T-Pigm and K-Pigm were developing with Tetra-primer ARMS-PCR and KASP technique, based on the specific nucleotide mutation in Pigm locus. Then, the consistency of the genotype and phenotype was evaluated in different varieties and the F2 population derived from Huaidao 9/ Gumei 4 by artificial inoculation methods of panicle blast.【Result】The sequence alignment analysis indicated that a specific SNP was at -515 bp of the Pigm-Nbs2 gene initiation codon in Gumei 4. The three different genotypes of Pigm locus could be distinguished by the Tetra-primer ARMS-PCR and KASP markers, which exactly conformed to the results of artificial inoculation.【Conclusion】 T-Pigm and K-Pigm markers could be used to detect the genotype of Pigm gene rapidly and accurately, and improved the selection efficiency of blast resistant rice varieties in breeding.
Tao CHEN, Xuchao SUN, Shanlei ZHANG, Wenhua LIANG, Lihui ZHOU, Qingyong ZHAO, Shu YAO, Ling ZHAO, Chunfang ZHAO, Zhen ZHU, Yadong ZHANG, Cailin WANG . Development and Verification of Specific Molecular Markers for Pigm Gene Associated with Broad-spectrum Resistance to Rice Blast[J]. Chinese Journal OF Rice Science, 2020 , 34(1) : 28 -36 . DOI: 10.16819/j.1001-7216.2020.9082
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