Chinese Journal OF Rice Science >
Identification and Genetic Analysis of Waxy Sparse Mutant wax1 in Rice
Received date: 2019-09-16
Revised date: 2019-10-27
Online published: 2020-01-10
【Objective】 Wax is an important protective substance on plant surface. Screening and identification of waxy-related mutants help elucidate the genetic mechanism of waxy synthesis in rice. 【Method】A waxy sparse mutant, waxy1, was isolated from an ethylmethylsulfone (EMS) mutagenic population of Xiangzaoxian 6. The morphological characteristics and agronomic traits of waxy1 mutant were analyzed. The F2 segregating population was constructed from a cross between waxy1 and japonica variety 02428, and the recessive individuals were selected for fine mapping of WAXY1 gene. In addition, the expressions of related genes were analyzed by quantitative PCR. 【Results】Compared with the wild type, wax1 mutant showed many morphological abnormalities, including small wrinkled leaves, shorter panicle and reduced waxy crystals on leaf surface. The wax1 mutant was also featured by increased effective panicle number, and obviously decreased plant height, 1000-grain weight and number of grains per panicle. Genetic analysis implied that the mutant phenotype was controlled by a pair of recessive nuclear gene. The WAXY1 gene was fine-mapped to a 49.8 kb interval between markers RM5806 and P1 on the chromosome 10. A total of 4 ORFs (open reading frames) were found in this region. Sequencing analysis indicated that a single base transition was present in the coding region of 3-ketoacyl-CoA synthase, which resulted in an amino acid substitution. In addition, mutation of WAXY1 increased the expression of KNOX gene OSH6, and caused differential expressions of some IAA genes. 【Conclusion】The WAX1 gene mutation resulted in the decrease of waxy crystals on leaf surface and abnormalities in plant growth and development through affecting shoot apex meristem and auxin signal transduction.
Key words： rice; waxy; gene mapping; auxin
Xiaowu PAN, Yongchao LI, Wenqiang LIU, Haibo XIONG, Zheng DONG, Dan XUE, Xinnian SHENG, Wenjin ZHAO, Xiucai WEI, Xiaoxiang LI . Identification and Genetic Analysis of Waxy Sparse Mutant wax1 in Rice[J]. Chinese Journal OF Rice Science, 2020 , 34(1) : 1 -7 . DOI: 10.16819/j.1001-7216.2019.9101
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