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Protective Effect of Glutaredoxin (AbGrx-1) on Aphelenchoides besseyi Under Oxidative Stress
Received date: 2019-01-28
Revised date: 2019-05-07
Online published: 2019-11-10
【Objective】 The antioxidant glutaredoxin (Grx) plays a crucial role in regulating intracellular redox homeostasis via scavenging of excess reactive oxygen species. The white-tip nematode Aphelenchoides besseyi can survive in adverse environments including high temperature, osmosis and oxidative stresses. To reveal the antioxidant function of Grx in A. besseyi, 【Method】 herein the full-length cDNA of glutaredoxin (named AbGrx-1) from A. besseyi was cloned by using rapid amplification of cDNA ends (RACE), and AbGrx-1 protein and evolutionary relationship were characterized; differential gene expression level was detected in the nematodes under oxidant and temperature stresses by using quantitative real-time PCR; the effect of the recombinant AbGrx-1 protein on A.besseyi survival was also tested.【Result】 The full-length AbGrx-1 cDNA contains a 5' UTR of 90, an ORF of 321 bp encoding a polypeptide of 106 amino acids and a 3' UTR of 97 bp. The deduced amino acid sequence of AbGrx-1 shares a high similarity with other nematodes’ Grxs, and the catalytic residue (CPYC) and glutathione binding sites (RSVP and GGDD) indicate that AbGrx-1 is categorized into Class I Grx. The phylogenetic tree showed AbGrx-1 is located in the same clade with the plant parasitic nematode Aphelenchus avenae. AbGrx-1 mRNA is highly induced in A. besseyi exposed to H2O2 solution and 12℃, but is suppressed in the nematodes at 0, 4, 37 and 45℃. The high concentration H2O2 solutions and high temperature are adverse to survival of A. besseyi, but the survival rate increases with the nematodes pre-soaked in AbGrx-1 recombinant protein solution. 【Conclusion】 AbGrx-1 is required for A. besseyi antioxidative immunity, and plays an essential role in overcoming oxidative damage and nematode survival.
Hui FENG, Yalei FAN, Jinfeng ZHANG, Hongli ZHU, Lihui WEI . Protective Effect of Glutaredoxin (AbGrx-1) on Aphelenchoides besseyi Under Oxidative Stress[J]. Chinese Journal OF Rice Science, 2019 , 33(6) : 565 -574 . DOI: 10.16819/j.1001-7216.2019.9016
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