Chinese Journal OF Rice Science >
Mechanism of Exogenous Brassinolide in Alleviating High Temperature Injury at Panicle Initiation Stage in Rice
Received date: 2019-03-29
Revised date: 2019-04-15
Online published: 2019-09-10
【Objective】The purpose of the study is to ascertain the effects of 2,4-epibrassinolide(EBR) application on rice panicle development and spikelet formation under high temperature, and to explore the physiological mechanism. 【Method】Heat-sensitive inbred rice cultivar IR36 was subjected to high temperature (HT, 40℃) and normal temperature (NT, 32℃) in climate chambers with EBR application, and the carbohydrate supplement, sucrose metabolism, cytokinin metabolism, and antioxidant capacity of young panicle were investigated. 【Result】1) EBR application increased the number of spikelets per panicle by 13.7% and 45.7% on average under HT and NT, respectively, in comparison to the control. And 0.15 mg/L EBR application level performed the highest efficiency in increasing the number of spikelet number, EBR application prompted panicle development and spikelet differentiation, and inhibited spikelet degeneration. 2) EBR application did not influence leaf net photosynthesis, meanwhile increased the dry matter and nonstructural carbohydrate accumulation in young panicles. Under high temperature, EBR application significantly upregulated the expression levels of sucrose transport genes OsSUT1, OsSUT2, and OsSUT4, and EBR enhanced the related enzymes activities of sucrose metabolism especially in HT than in NT. 3) EBR application reduced the expression levels of cytokinin oxidase genes, OsCKX5 and OsCKX9, under HT. Meanwhile, EBR application promoted the genes expression involved in cytokinin biosynthesis and signal transduction both under HT and NT treatments. 4) EBR application increased the activities of superoxide dismutase, catalase and peroxidase, reduced the content of superoxide under HT. 【Conclusion】Under HT conditions, EBR application at appropriate concentration could alleviate heat damage to spikelet formation by promoting carbohydrates transportation to young panicles, inhibiting cytokinin oxidation, and reducing peroxidation injures. Meanwhile, EBR application could promote spikelet formation under normal temperature.
Yanhua CHEN, Yaliang WANG, Defeng ZHU, Qinghua SHI, Huizhe CHEN, Jing XIANG, Yikai ZHANG, Yuping ZHANG . Mechanism of Exogenous Brassinolide in Alleviating High Temperature Injury at Panicle Initiation Stage in Rice[J]. Chinese Journal OF Rice Science, 2019 , 33(5) : 457 -466 . DOI: 10.16819/j.1001-7216.2019.9036
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