Chinese Journal OF Rice Science >
Influence of DNA Methylation Inhibitor 5-Aza-2′-deoxycytidine on DNA Methylation and Seedling Development of Rice
Received date: 2019-01-01
Revised date: 2019-01-15
Online published: 2019-03-10
【Objective】 DNA methylation is an epigenetic modification broadly existed in higher plants. DNA methylation plays an important role in regulating gene expression, maintaining the genomic stability and modulating plant development. Here, we used a DNA methylation inhibitor, 5-Aza-2′-deoxycytidine (AZA), to study how the DNA methylation status influences rice development. 【Method】We applied AZA to the rice seedlings to explore its influence on seedling development. 【Results】AZA-treatment resulted in overall hypomethylation of the rice genome. AZA-treated plants were strikingly shorter than the control, indicating AZA treatment disturbed normal development of the plants. However, the seed germination was not significantly affected. Expression of several key genes involved in DNA methylation could be repressed by AZA treatment. In addition, some genes involving defense and photosynthesis pathways were also down-regulated. 【Conclusion】Our findings confirm that AZA is a potent DNA methylation inhibitor and suggest that to maintain proper DNA methylation status is important for rice development.
Key words： DNA methylation; seedling growth; gene expression; rice
Hui DENG, Zhiguo E, Baixiao NIU, Lei WANG, Chen CHEN . Influence of DNA Methylation Inhibitor 5-Aza-2′-deoxycytidine on DNA Methylation and Seedling Development of Rice[J]. Chinese Journal OF Rice Science, 2019 , 33(2) : 108 -117 . DOI: 10.16819/j.1001-7216.2019.9001
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