Chinese Journal OF Rice Science >
Development of Specific Markers for Pigm in Marker-Assisted Breeding of Panicle Blast Resistant japonica Rice
Received date: 2017-11-09
Revised date: 2018-01-30
Online published: 2018-09-10
【Objective】 Pigm is a broad-spectrum resistance gene to rice blast, which is isolated from Gumei 4, a variety with durable resistance. Pigm is allelic to Piz, Piz-t, Pi2, Pi9, Pi40 and so on, but shows different resistance spectrum. To breed resistance variety carrying Pigm with molecular marker-assisted selection, discovering Pigm-tightly-linked markers is important.【Method】We identified a unique DNA sequence of Gumei 4 by genomic sequencing, and developed a set of specific markers tagged to Pigm base on the sequence divergence. Then, by means of molecular-assisted selection and background gene screening, Pigm was pyramided to three representative japonica varieties belonging to different ecotypes from Yangtze-Huai River Area. 【Result】 An InDel marker Pigm-4 which is closely linked to the functional unit of Pigm was developed. Pigm-4 showed distinct specificity by genotype screening of various rice varieties, and can distinguish the genotype of Pigm from its alleles Piz, Piz-t, Pi2, Pi9 and Pi40. Although receptors carry with resistant genes such as Pib+Pi54 (
Key words： Pigm; specific-marker; japonica rice; panicle blast; resistance
Shengyuan ZENG, Chuang LI, Cancan DU, Liting SUN, Dedao JING, Tianzi LIN, Bo YU, Huafei QIAN, Weicheng YAO, Yiwen ZHOU, Hongbing GONG . Development of Specific Markers for Pigm in Marker-Assisted Breeding of Panicle Blast Resistant japonica Rice[J]. Chinese Journal OF Rice Science, 2018 , 32(5) : 453 -461 . DOI: 10.16819/j.1001-7216.2018.7135
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