Chinese Journal OF Rice Science >
Functional Analysis of a Copper/Zinc SOD Encoding Gene in Response to Arsenite Stress in Rice
Received date: 2017-12-25
Revised date: 2018-02-01
Online published: 2018-09-10
【Objective】Os08g44770.1 encodes a Cu/Zn-SOD protein in rice, but its biological function in response to arsenite [As(Ⅲ)] remains unclear. The results presented here would be intriguing for further investigating the molecular mechanisms of Os08g44770.1-mediated arsenic tolerance in rice, and also providing useful new idea for rice stress resistance breeding. 【Methods】Using wild type(WT) Nipponbare and two transgenic rice lines overexpressing Os08g44770.1 as materials, we investigated the phenotypes of WT and transgenic plants after exposure to As(Ⅲ), and further uncovered its physiological and molecular mechanisms in regulation of As(Ⅲ) tolerance in rice through stress treatment, physiological index measurement, and gene expression analysis, etc. 【Result】The results showed that, compared with WT, transgenic rice plants were more sensitive to As(Ⅲ) stress; Under As(Ⅲ) exposure, transgenic plants showed shorter relative root elongation, lower biomass (dry weight) and chlorophyll content, as well as lower root cell membrane integrity and antioxidant abilities in leaves. Moreover, Os08g44770.1 exhibited slightly different expression patterns but both showing expression peak at 24 h after As(Ⅲ) treatment in leaves of WT and transgenic plants. 【Conclusion】Strong overexpression of Os08g44770.1 in rice would result in its hypersensitivity to arsenite stress.
Key words： rice; Cu/Zn SOD; arsenite; transgenic; physiological mechanism; gene expression
Lingling DOU, Haichao HU, Long MA, Xiaonan KE, Mingyue LIU, Wangmin LIAN, Ke JIN, Lingjuan XIE, Qingpo LIU . Functional Analysis of a Copper/Zinc SOD Encoding Gene in Response to Arsenite Stress in Rice[J]. Chinese Journal OF Rice Science, 2018 , 32(5) : 437 -444 . DOI: 10.16819/j.1001-7216.2018.7154
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