Chinese Journal OF Rice Science >
Expression Patterns and Regulation of Transcription Factor Gene OsSHR2 in Vegetative Growth in Rice
Received date: 2017-03-30
Revised date: 2017-08-18
Online published: 2018-09-10
【Objective】OsSHR2(LOC_Os03g31880) is a homologous gene of AtSHR of Arabidopsis thaliana, which falls into GRAS transcription factor family together with OsSHR1, OsSCR1 and OsSCR2 in rice. It has been reported that the transcription factor genes SHR and SCR regulate the development of roots and leaves, and participate in various life activities. We analyzed the temporal and spatial expression patterns and the way OsSHR2 regulates vegetative growth in rice.【Method】The function of OsSHR2 was verified by bioinformatics analysis, expression pattern analysis, germination kinetic analysis and hydroponic experiments.【Result】 Biomechanical analysis showed that OsSHR2, OsSHR1, OsSCR1 and OsSCR2 had high homology with SHR subfamily and SCR subfamily in Arabidopsis thaliana and other species. The expression pattern analysis by qRT-PCR and pOsSHR2::GUS staining showed that OsSHR2 was strongly expressed in the roots, leaves, vascular tissues and reproductive organs during the whole vegetative and reproductive growth stages, and especially in the stele of the root tip, lateral root primordium and the central of leaf and stem vascular tissue. Additionally, the relative expression of OsSHR2 was down-regulated in Pi-deficiency in shoots and roots of wild type. The seed germination and hydroponic experiment analysis of CRISPR-Cas9 mutant osshr2 showed that seed germination of osshr2 was delayed and with lower germination rate than that of WT, in addition the length of shoot and root of osshr2 were significantly shorter than WT under Pi-sufficient and Pi-deficient conditions.【Conclusion】OsSHR2 plays an important role in the development of shoots and roots, the formation of vascular tissue and various activity in vegetative and reproductive growth, which lays an important theoretical basis for the application of OsSHR2 in molecular breeding.
Key words： rice; OsSHR2; temporal and spatial expression pattern; vegetative growth; phosphorus
Zhantian ZHANG, Yafei SUN, Hao AI, Wenzhen LUO, Bing FENG, Wenxian SUN, Guohua XU, Shubin SUN . Expression Patterns and Regulation of Transcription Factor Gene OsSHR2 in Vegetative Growth in Rice[J]. Chinese Journal OF Rice Science, 2018 , 32(5) : 427 -436 . DOI: 10.16819/j.1001-7216.2018.7037
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