Chinese Journal OF Rice Science >
Protective Roles of Over-expression of OsXDH in Rice Seedlings Under High Temperature Stress
Online published: 2018-07-10
【Objective】Xanthine dehydrogenase (XDH) is considered as a key enzyme in purine metabolism. This paper aims to reveal the physiological mechanism of XDH to relieve high temperature stress by analyzing the physiological indexes of rice seedling leaves differed in expression level of OsXDH under high temperature stress. 【Method】In this work, chlorophyll content, relative water content, soluble protein content, reactive oxygen metabolism, antioxidant enzyme activities, XDH activity, allantoin and allantoate contents were measured under high temperature stress in OsXDH over-expressed transgenic lines and wild type during seedling stage. 【Result】The results revealed that there were no significant difference between wild type and transgenic lines in all tested physiological parameters except XDH activity and ureides (allantoin and allantoate) content before high temperature stress. After high temperature stress for 5 days, compared with the transgenic lines, seedlings of wild type showed significantly decreased chlorophyll content, relative water content, soluble protein content, and antioxidant enzyme activities, but significantly increased contents of H2O2 and Malondialdehyde(MDA). As we know that chlorophyll content, relative water content, and soluble protein content all increased in transgenic lines and wild type after post-stress recovery, especially in transgenic lines. H2O2 and MDA content all decreased. For transgenic lines, these indexes were lower than those in wild type. The XDH enzyme activity and purine metabolites allantoin and allantoate contents in the wild type and transgenic lines were induced by high temperature. OsXDH over-expressed transgenic lines had significantly higher XDH enzyme activity and allantoin and allantoate contents compared with wild type throughout the process. 【Conclusion】XDH can effectively improve the tolerance of rice seedlings to high temperature stress by regulating the synthesis of ureides, compensating their antioxidant capacity and enhancing the activities of antioxidant enzymes.
Ruicai HAN, Ruqi SU, Jianlin WAN, Qizhang LONG, Yongjun ZENG, Xiaohua PAN, Qinghua SHI, Ziming WU . Protective Roles of Over-expression of OsXDH in Rice Seedlings Under High Temperature Stress[J]. Chinese Journal OF Rice Science, 2018 , 32(4) : 365 -373 . DOI: 10.16819/j.1001-7216.2018.7130
| [1] | Challinor A J, Ewert F, Arnold S, Simelton E, Fraser E.Crops and climate change: Progress, trends, and challenges in simulating impacts and informing adaptation.J Exp Bot, 2009, 60(10): 2775-2789. |
| [2] | Maestri E, Klueva N, Perrotta C, Gulli M, Nguyen H T, Marmiroli N.Molecular genetics of heat tolerance and heat shock proteins in cereals.Plant Mol Biol, 2002, 48(5-6): 667-681. |
| [3] | 段骅, 杨建昌. 高温对水稻的影响及其机制的研究进展. 中国水稻科学, 2012, 26(4): 393-400. |
| [3] | Duan Y, Yang J C.Research advances in the effect of high temperature on rice and its mechanism. Chin J Rice Sci, 2012, 26(4): 393-400.(in Chinese with English abstract) |
| [4] | 曹云英, 赵华. 高温胁迫下油菜素内酯对水稻幼苗的保护作用. 中国水稻科学, 2007, 21(5):525-529. |
| [4] | Cao Y Y, Zhao H.Protective Roles of brassinolide in rice seedlings under heat stress. Chin J Rice Sci, 2007, 21(5): 525-529. (in Chinese with English abstract) |
| [5] | 周伟辉, 薛大伟, 张国平. 高温胁迫下水稻叶片的蛋白响应及其基因型和生育期差异. 作物学报, 2011, 37(5): 820-831. |
| [5] | Zhou W Hui, Xue D W, Zhang G P.Protein response of rice leaves to high temperature stress and its difference of genotypes at different growth stage.Acta Agron Sin, 2011, 37(5): 820-831.(in Chinese with English abstract) |
| [6] | 曹云英, 段骅, 杨立年, 王志琴, 周少川, 杨建昌. 减数分裂期高温胁迫对耐热性不同水稻品种产量的影响及其生理原因. 作物学报, 2008, 34(12): 2134-2142. |
| [6] | Cao Y Y, Duan H, Yang L N, Wang Z Q, Zhou S C, Yang J C.Effect of heat-stress during meiosis on grain yield of rice cultivars differing in heat-tolerance and its physiological mechanism.Acta Agron Sin, 2008, 34(12): 2134-2142. (in Chinese with English abstract) |
| [7] | 张桂莲, 张顺堂, 王力, 肖应辉, 唐文帮, 陈光辉, 陈立云. 抽穗结实期不同时段高温对稻米品质的影响. 中国农业科学, 2013, 46(14): 2869-2879. |
| [7] | Zhang G L, Zhang S T, Wang L, Xiao Y H, Tang W B, Chen G H, Chen L Y.Effects of high temperature at different times during the heading and filling periods on rice quality.Sci Agric Sin, 2013, 46(14): 2869-2879.(in Chinese with English abstract) |
| [8] | Werner A K, Witte C P.The biochemistry of nitrogen mobilization: purine ring catabolism.Trends Plant Sci, 2011, 16(7): 381-387. |
| [9] | Yesbergenova Z, Yang G H, Oron E, Soffer D, Fluhr R, Sagi M.The plant Mo-hydroxylases aldehyde oxidase and xanthine dehydrogenase have distinct reactive oxygen species signatures and are induced by drought and abscisic acid. Plant J, 2005, 42(6): 862-876. |
| [10] | Zdunek-Zastocka E, Lips H S.Is xanthine dehydrogenase involved in response of pea plants (Pisum sativum L.) to salinity or ammonium treatment? Acta Physiol Plant, 2003, 25(4): 395-401. |
| [11] | Barabás N K, Omarov R T, Erdei L, Lips S H.Distribution of the Mo-enzymes aldehyde oxidase, xanthine dehydrogenase and nitrate reductase in maize (Zea mays L.) nodal roots as affected by nitrogen and salinity. Plant Sci, 2000, 155(1): 49-58. |
| [12] | You S H, Zhu B, Wang F B, Han H J, Sun M, Zhu H W, Peng R H, Yao Q H.A Vitis vinifera xanthine dehydrogenase gene, VvXDH, enhances salinity tolerance in transgenic Arabidopsis. Plant Biotechnol Rep, 2017, 11(4): 247. |
| [13] | Hofmann N R.Opposing functions for plant xanthine dehydrogenase in response to powdery mildew infection: production and scavenging of reactive oxygen species.Plant Cell, 2016, 28(5): 1001. |
| [14] | 孙学成, 胡承孝. 高等植物含钼酶与钼营养. 植物生理学通讯, 2005, 6(3): 395-399. |
| [14] | Sun X C, Hu C X.Molybdoenzymes and Molybdenum Nutrition in Higher Plants.Plant Physiol Commun, 2005, 6(3): 395-399. (in Chinese with English abstract) |
| [15] | Taylor N J, Cowan A K.Xanthine dehydrogenase and aldehyde oxidase impact plant hormone homeostasis and affect fruit size in ‘Hass’ avocado.J Plant Res, 2004, 117(2): 121-130. |
| [16] | Lichtenthaler H K, Wellbuen A R.Determinations of total caroten oids and chlorophylls a and b leaf extracts in different solvents.Biochem Soc Trans, 1983, 11(5): 591-592. |
| [17] | 张志良. 植物生理学实验指导. 2版. 北京: 高等教育出版社, 1991: 183-184. |
| [17] | Zhang Z L.Experimental Guidance on Plant Physiology. 2nd ed. Beijing: Higher Education Press, 1991: 183-184. (in Chinese) |
| [18] | 李合生, 孙群, 赵世杰, 章文华. 植物生理生化实验原理与技术. 北京: 高等教育出版社, 2000. |
| [18] | Li H S, Sun Q, Zhao S J, Zhang W H.The Experiment Principle and Technique on Plant Physiology and Biochemistry. Beijing: Higher Education Press, 2000. (in Chinese) |
| [19] | Lin C C, Kao C H.Abscisic acid induced changes in cell wall peroxidase activity and hydrogen peroxide level in roots of rice seedlings.Plant Sci, 2001, 160: 323-329. |
| [20] | Sagi M, Omarov R T, Lips S H.The Mo-hydroxylases xanthine dehydrogenase and aldehyde oxidase in ryegrass as affected by nitrogen and salinity.Plant Sci, 1998, 135(2): 125-135. |
| [21] | 张桂莲, 陈立云, 雷东阳, 张顺堂. 水稻耐热性研究进展. 杂交水稻, 2005, 20(1): 1-5. |
| [21] | Zhang G L, Chen L Y, Lei D Y, Zhang S T.Research progress on heat resistance of rice. Hybrid Rice, 2005, 20(1): 1-5.(in Chinese) |
| [22] | 李轶冰, 杨顺强, 任广鑫, 冯永忠, 张强, 李鹏. 低温处理下不同禾本科牧草的生理变化及其抗寒性比较. 生态学报, 2009, 29(3): 1341-1347. |
| [22] | Li T B, Yang S Q, Ren G X, Feng Y Z, Zhang Q, Li P.Changes analysis in physiological properties of several gram ineous grass species and cold-resistance comparison on under cold stress.Acta Ecol Sin, 2009, 29(3): 1341-1347. (in Chinese with English abstract) |
| [23] | 张顺堂, 张桂莲, 陈立云, 肖应辉. 高温胁迫对水稻剑叶净光合速率和叶绿素荧光参数的影响. 中国水稻科学, 2011, 25(3): 335-338. |
| [23] | Zhang S T, Zhang G L, Chen L Y, Xiao Y H.Effects of high temperature stress on net photosynthetic rate and chlorophyll fluorescence parameters of flag leaf in rice.Chin J Rice Sci, 2011, 25(3): 335-338. (in Chinese with English abstract) |
| [24] | Rang Z W, Jagadish S V K, Zhou Q M, Craufurd P Q, Heuer S. Effect of high temperature and water stress on pollen germination and spikelet fertility in rice.Environ Exp Bot, 2011, 70(1): 60-65. |
| [25] | Mehdy M C.Active oxygen species in plant defense against pathogens.Plant Physiol, 1994, 105(2): 467-472. |
| [26] | Gill S S, Tuteja N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants.Plant Physiol Bioch, 2010, 48(12): 909-930. |
| [27] | 王贺正, 马均, 李旭毅, 李艳, 张荣萍, 汪仁全. 水分胁迫对水稻结实期活性氧产生和保护系统的影响. 中国农业科学, 2007, 40(7): 1379-1387. |
| [27] | Wang H Z, Ma J, Li X Y, Li Y, Zhang R P, Wang R Q.Effects of Water Stress on Active Oxygen Generation and Protection System in Rice During Grain Filling Stage.Sci Agric Sin, 2007, 40(7): 1379-1387. (in Chinese with English abstract) |
| [28] | 杨淑慎, 高俊凤. 活性氧、自由基与植物的衰老. 西北植物学报, 2001, 21(2): 215-220. |
| [28] | Yang S S, Gao J F.Influence of active oxygen and free radicals on plant senescence.Acta Bot Boreal, 2001, 21(2): 215-220. (in Chinese with English abstract) |
| [29] | Hung K T, Kao C H.Nitric oxide counteracts the senescence of rice leaves induced by abscisic acid.J Plant Physiol, 2003, 160(8): 871-879. |
| [30] | Nakagawa A, Sakamoto S, Takahashi M, Morikawa H, Sakamoto A.The RNAi-mediated silencing of xanthine dehydrogenase impairs growth and fertility and accelerates leaf senescence in transgenic Arabidopsis Plants. Plant Cell Physiol, 2007, 48(10): 1484-1495. |
| [31] | Brychkova G, Alikulov Z, Fluhr R, Sagi M.A critical role for ureides in dark and senescence-induced purine remobilization is unmasked in the Atxdh1 Arabidopsis mutant. Plant J, 2008, 54(3): 496-509. |
| [32] | Watanabe S, Nakagawa A, Izumi S, Shimada H, Sakamoto A.RNA interference-mediated suppression of xanthine dehydrogenase reveals the role of purine metabolism in drought tolerance in Arabidopsis. FEBS Lett, 2010, 584(6): 1181-1186. |
| [33] | Pastori G M, del Rio L A. Natural senescence of pea leaves: an activated oxygen-mediated function for peroxisomes.Plant Physiol, 1997, 113(2): 411-418. |
| [34] | Yobi A, Wone B W, Xu W X, Alexander D C, Guo L N, Ryals J A, Oliver M J, Cushman J C.Metabolomic profling in Selaginella lepidophylla at various hydration states provides new insights into the mechanistic basis of desiccation tolerance. Mol Plant, 2013, 6(2): 369-385. |
| [35] | Brychkova G, Fluhr R, Sagi M.Formation of xanthine and the use of purine metabolites as a nitrogen source in Arabidopsis plants. Plant Signal & Behav, 2008, 3(11): 999-1001. |
| [36] | 郭培国, 李荣华. 夜间高温胁迫对水稻叶片光合机构的影响. 植物学报, 2000, 42(7): 673-678. |
| [36] | Guo P G, Li R H.Effects of high nocturnal temperature on photosynthetic organization in rice leaves.Acta Bot Sin, 2000, 42(7): 673-678. (in Chinese with English abstract) |
| [37] | Ma X F, Wang W M, Bittner F, Schmidt N, Berkey R, Zhang L L, King H, Zhang Y, Feng J Y, Wen Y Q, Tan L Q, Li Y, Zhang Q, Deng Z N, Xiong X Y, Xiao S Y.Dual and opposing roles of xanthine dehydrogenase in defense-associated reactive oxygen species metabolism in Arabidopsis. Plant Cell, 2016, 28(5): 1108-1126. |
| [38] | Smith P M C, Atkins C A. Purine biosynthesis, big in cell division, even bigger in nitrogen assimilation.Plant Physiol, 2004, 128(3): 793-802. |
| [39] | Bittner F, Oreb M, Mendel R R.ABA3 is a molybdenum cofactor sulfurase required for activation of aldehyde oxidase and xanthine dehydrogenase in Arabidopsis thaliana. J Biol Chem, 2001, 276(44): 40381-40384. |
| [40] | Watanabe S, Kounosu Y, Shimada H, Sakamoto A.Arabidopsis xanthine dehydrogenase mutants defective in purine degradation show a compromised protective response to drought and oxidative stress. Plant Biotechnol,2014, 31(2): 173-178. |
| [41] | Leydecker M T, Moureaux T, Kraepiel Y, Caboche M.Molybdenum cofactor mutants, specifically impaired in xanthine dehydrogenase activity and abscisic acid biosynthesis, simultaneously overexpress nitrate reductase. Plant Physiol, 1995, 107(4): 1427-1431. |
| [42] | Taylor N, Cowan K.Plant hormone homeostasis and the control of avocado fruit size.Plant Grow Regul, 2001, 35(3): 247-255. |
/
| 〈 |
|
〉 |