Chinese Journal OF Rice Science >
Identification and Genetic Analysis of Thermo-sensitive Mutant osv15 in Rice
Received date: 2017-11-01
Revised date: 2018-02-06
Online published: 2018-07-10
：【Objective】The objective of the research is to identify and clone a new thermo-sensitive gene of rice, and to reveal the molecular mechanism involved in the process of chloroplast development and photosynthesis, providing theoretical support for photosynthetic efficiency breeding. 【Method】A yellow leaf mutant osv15 was obtained from rice cultivar Nipponbare (abbreviated as Nip) mutagenized by irradiation. The phenotypic, agronomic traits and genetic patterns of osv15 were analyzed in detail, and F2 population was constructed by crossing the mutant with Kasalath, and mutant individuals were selected to map the gene OsV15.【Result】The mutant osv15 exhibited etiolation at seedling stage, with only ten percent of the chlorophyll content of the wild type, declined photochemical efficiency and abnormal structure of chloroplasts under low temperature condition (22℃). As temperature rose, leaf color of the mutant regreened due to increased chlorophyll content, which recovered to sixty-eight percent of that of the wild type under higher temperature condition (30℃). In natural environment, osv15 showed leaf yellowing from seedling stage to maturity stage, and significant differences in plant height, tiller number, seed setting rate and so on. Genetic analysis revealed that the mutant phenotype of osv15 was controlled by a single recessive gene. The gene was located in the 84 kb region of chromosome 6, between molecular markers S4 and S5. Sequencing analysis in this region showed that Cpn60β1(LOC_Os06g02380) that encodes a putative chaperonin 60 beta precursor, carried a single base deletion in osv15.【Conclusion】osv15 is a new thermo-sensitive mutant, and Cpn60β1 may be the mutant gene.
Key words： rice; thermo-sensitive; chloroplast; gene mapping
Yuehan QI, Ruili LI, Fang WANG, Hongjia LIU, Keke YI, Cheng ZHU . Identification and Genetic Analysis of Thermo-sensitive Mutant osv15 in Rice[J]. Chinese Journal OF Rice Science, 2018 , 32(4) : 349 -356 . DOI: 10.16819/j.1001-7216.2018.7131
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