Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2018 , Vol. 1 >Issue 1: 189 - 199

DOI: https://doi.org/10.16819/j.1001-7216.2018.7107

Research Papers

Scanning Electron Microscopic Analysis of Grain Cross-section from Rice with Different Transparency

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  • 1Jiangsu Key Laboratory for Crop Genetics and Physiology / Key Laboratory of Plant Functional Genomics of Ministry of Education / Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou225009, China
    2Instrumental Analysis Center, Yangzhou University, Yangzhou 225009, China
    3College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
*Corresponding author, E-mail: qqliu@yzu.edu.cn

Received date: 2017-09-01

  Online published: 2018-03-10

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Abstract

【Objective】 The objective of this study was to set up an ideal method of observation of a single starch granule cross-section, to clarify the relationship between rice transparency and amylose content and to find the structure foundation that caused the poor rice transparency. 【Method】 The pre-treated rice grain cross-section and isolated starch were observed under a scanning electron microscopy.【Result】The transparency of both indica and japonica soft rice with amylose contentbelow 15%was significantly decreased. By comparingdifferent methods of grain cross-section pre-treatments, we found that the direct mechanical fracture method can’t break a single starch granule and the mechanical fracture with glass scraping method could break down an individual starch granule, however, it will cause rough surface and thus difficult to be analyzed quantitatively. As for the liquid nitrogen fracture method, we found it’s an ideal way to break a single starch granule and the cavity in starch granule can be easily observed. We then further analyzed the starch granule arrangement and the internal structure of an individual starch granule in paddy rice from both indica and japonica rice cultivars. We found that all the starch granules arranged tightly and obvious air containing space was observed in an individual starch granule from grains of all dark endosperm rice. Also, we found that the number and area of starch granule cavity in glutinous rice was significantly higher than those in dark endosperm rice. Furthermore, we proved that the number and area of starch granule cavity increased as the grain moisture content decreased.【Conclusion】The direct liquid nitrogen fracture treatment of rice grain cross-section is an ideal method for the single starch granule cross-section observation. Besides, the rice grain transparency showed a close relationship with the moisture content and also the number and size of cavity in the middle of a starch granule but not the starch granule arrangement. Moreover, the size and number of cavities increased as the amylose content decreased.

Key words： Oryza sativa L.; starch; scanning electron microscopy; transparency; cavity

Cite this article

Yan LU, Xiaomin ZHANG, Yan QI, Changquan ZHANG, Yuping LING, Qiaoquan LIU . Scanning Electron Microscopic Analysis of Grain Cross-section from Rice with Different Transparency[J]. Chinese Journal OF Rice Science, 2018 , 1(1) : 189 -199 . DOI: 10.16819/j.1001-7216.2018.7107

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