Chinese Journal OF Rice Science >
Expression Under Hypertonic Osmotic Stress of Ab-lea from Aphelenchoides besseyi
Online published: 2017-11-10
【Objective】Rice white tip nematodes (Aphelenchoides besseyi) can enter osmobiosis to survive hypertonic osmotic stress in soil and seeds before infecting hosts, making the prevention and control of the nematode rather difficult. Normally, under hypertonic osmotic stress, the gene expression of late embryogenesis abundant protein (LEA) will significantly increase. As an adversity expressive protein, LEA can protect the organism under adversity. In order to study the expression pattern and the function of LEA gene from A. besseyi (Ab-lea) under hypertonic osmotic stress, 【Method】the gene was cloned according to the results of the transcriptome sequencing. A saturated MgSO4·7H2O solution was used as a hypertonic osmotic solution to dehydrate nematodes. The expression of Ab-lea was tested by RT-PCR during Osmobiosis. RNAi was used to investigate the survival of nematodes under hypertonic osmotic stress after silencing of Ablea.【Result】Nematodes will turn into osmobiosis after soaking in a saturated MgSO4·7H2O solution for 240 min. RT-PCR results showed that the expression of Ab-lea was upregulated when nematodes were at the beginning of hypertonic osmotic stress treatment and during the osmobiosis. 【Conclusion】The survival rate of the nematodes dropped significantly under osmobiosis after Ab-lea silence, which showed that Ab-lea may be involved in the regulation of osmobiosis of the nematode. It lays a foundation of further exploration for the physiological mechanism of nematode-resistance to hypertonic osmotic stress and provides new ideas for the prevention and control of the nematode.
Qiaoli CHEN, Feng WANG, Danlei LI, Yaming LING, Ruizhi ZHANG . Expression Under Hypertonic Osmotic Stress of Ab-lea from Aphelenchoides besseyi[J]. Chinese Journal OF Rice Science, 2017 , 31(6) : 652 -657 . DOI: 10.16819/j.1001-7216.2017.7004
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