Chinese Journal OF Rice Science >
Identification and Fine Mapping of Small Grain Gene SG101 in Rice (Oryza sativa L.)
Received date: 2017-11-05
Revised date: 2017-04-03
Online published: 2017-11-10
【Objective】Grain size is an important agronomic trait in determining grain yield. The characterization and identification of grain size related genes will be beneficial to expound the genetic regulatory mechanisms behind yield formation in rice. 【Method】Here, we report the characterization of a rice (Oryza sativa L.) mutant, small grain 101 (sg101), gained by EMS mutagenesis for Zhonghua 11. The effects of sg101 on grain size, panicle-related traits, cell number and size of lemma were assessed by morphological and cytological methods. The responses to brassinolide(BR) were detected based on the variation in leaf angle and coleoptile length. The expression level of BR signal and synthesis related genes were tested by RT-PCR. Map-based cloning was executed for fine mapping of SG101.【Result】The grain length, grain width and grain weight were significantly reduced in sg101. Moreover, sg101 showed decreased panicle length, secondary rachis branch number, plant height and seed setting rate. The paraffin section observation under a scanning electron microscope indicated that the cell number in sg101 was significantly decreased, while the cell size was similar to wild type, suggesting that SG101 affected the grain size by regulating the cell division. Quantitative RT-PCR analysis displayed that the expression of cell cycle related genes was reduced in sg101. After treatment with BR, sg101 showed smaller leaf angle and shorter coleoptile than those of the wild type and the expression of BR biosynthetic and signal pathway related genes was down-regulated in sg101. Genetic analysis revealed that sg101 phenotype was controlled by a single recessive nuclear gene.【Conclusion】SG101 was mapped on the long arm of chromosome 1 between two STS markers S5 and S6 with a 265 kb physical distance. It laid a base for the further cloning and functional analysis of SG101.
Key words： rice; small grain; sg101; brassinolide
Yuqiong WANG, Yaolong YANG, Yujia LENG, Lichao HUANG, Long CHEN, Liping DAI, Zhengjun TU, Yihong GAO, Jiang HU, Li ZHU, Guangheng ZHANG, Deyong REN, Zhenyu GAO, Guojun DONG, Guang CHEN, Longbiao GUO, Guoyou YE, Qian QIAN, Dali ZENG . Identification and Fine Mapping of Small Grain Gene SG101 in Rice (Oryza sativa L.)[J]. Chinese Journal OF Rice Science, 2017 , 31(6) : 580 -589 . DOI: 10.16819/j.1001-7216.2017.6152
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