Chinese Journal OF Rice Science >
Induced Expression Profiles of Hsp70s in Brown Planthoppers, Nilaparvata lugens, Under Different Temperatures
Received date: 2016-12-23
Revised date: 2017-02-17
Online published: 2017-09-10
【Objective】The brown planthopper (BPH), Nilaparvata lugens, is one of the major insect pests on rice in China. In recent years, the overwintering area of BPH expanded with global warming. In order to explore the response mechanisms of BPH to temperature, the hsp70s were cloned and the induced expression profiles of these genes were analyzed. 【Method】 The nucleotide sequences of hsp70s were gotten from transcriptome sequencing and GenBank database, the phylogenetic tree of HSP70 was based on neighbor-joining method by MEGA4.1, and the induced expression profiles were analyzed by real-time quantitative PCR. 【Result】 It was found that fifteen hsp70 homologous genes with complete open reading frame were gotten in BPH, and 8 out of 15 genes were expressed in normal conditions or temperature stress. These eight hsp70s were distributed in the cytoplasm/nucleus, endoplasmic reticulum or mitochondria, and had significantly different expression in normal conditions. After 30-44°C high temperature treatment, the expression levels of KX976471, KX976473, KX976475, KX976476, KX976477 and KX976478 were up-regulated, and the maximum increased times was 1.72-245.33. However, only the expression level of KX976475 increased by 2.38 times, the other seven were not changed or even significantly decreased in expression level after 0-22°C temperature treatment.【Conclusion】It was found that eight hsp70s were expressed in normal conditions or temperature stress in the cytoplasm/nucleus, endoplasmic reticulum or mitochondria. These hsp70s might play an important role in high temperature adaptation, but a limited role in cold adaptation in BPH.
Key words： Nilaparvata lugens; temperature; heat shock protein 70; induced expression
Dan SHAN, Lihua WANG, Yueliang ZHANG, Yangchun HAN, Hongtao NIU, Lei PAN, Jichao FANG . Induced Expression Profiles of Hsp70s in Brown Planthoppers, Nilaparvata lugens, Under Different Temperatures[J]. Chinese Journal OF Rice Science, 2017 , 31(5) : 533 -541 . DOI: 10.16819/j.1001-7216.2017.6171 533
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