Chinese Journal OF Rice Science >
Mapping of QTLs for Heading Date Using Whole-genome Re-sequenced Chromosome Segment Substitution Lines in Rice
Received date: 2017-02-08
Revised date: 2017-03-08
Online published: 2017-07-10
【Objective】Heading date is an important agronomic trait in rice, it is a typical quantitative trait controlled by multiple genes. As novel research material, chromosome segment substitution lines are useful in QTL fine mapping and cloning because of minimizing the interference of genetic background among plants. 【Method】In this study, 128 whole-genome re-sequenced chromosome segment substitution lines derived from Nipponbare as donor parent in the background of 9311, were used for mapping QTLs for heading date by combining the sequencing-based Bin-map with multiple linear regression analysis. 【Result】Six QTLs for heading date were identified in two environments and two years. qHD2.1 was mapped in the region of 759 848 bp on the chromosome 2; qHD2.2 was mapped in the region of 45 286 bp on the chromosome 2; qHD 3.1 was mapped in the region of 147 931 bp on the chromosome 3; qHD5.1 was mapped in the region of 213 351 bp on the chromosome 5; qHD5.2 was mapped in the region of 442 305 bp on the chromosome 5; qHD8.1 was mapped in the region of 538 176 bp on the chromosome 8. 【Conclusion】The results are important for the QTLs cloning and provide a foundation for understanding molecular regulation mechanism of heading date in rice.
Key words： rice; chromosome segment substitution lines; re-sequenced; heading date
Jun WANG, Jinyan ZHU, Yajun TAO, Yong ZHOU, Fangjun FAN, Wenqi LI, Fangquan WANG, Weigong ZHONG, Jie YANG, Guohua LIANG . Mapping of QTLs for Heading Date Using Whole-genome Re-sequenced Chromosome Segment Substitution Lines in Rice[J]. Chinese Journal OF Rice Science, 2017 , 31(4) : 364 -370 . DOI: 10.16819/j.1001-7216.2017.7017
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