Chinese Journal OF Rice Science >
AFLP Analyses of Genetic Diversity and Population Genetic Structure of Magnaporthe oryzae from South China Crop Breeding Area
Received date: 2016-10-20
Revised date: 2016-11-30
Online published: 2017-05-10
【Objective】 In order to clarify the genetic differentiation of Magnaporthe oryzae in South China Crop Breeding Area in Hainan Province, 【Method】the genetic diversity and population genetic structure of 60 Magnaporthe oryzae isolates collected from the core region (Sanya, Ledong and Baoting) and non-core region (Qiongzhong, Tunchang and Ding’an) of South China Crop Breeding Area were comparatively analyzed using amplified fragment length polymorphism(AFLP) technique. 【Result】The cluster analysis showed that almost all isolates were clustered in one group, and there was no obvious subgroup. The analysis of population genetic structure showed that the percentage of polymorphic loci(PPL), Shannon’s information index(I) and gene flow(Nm) in the core population were 87.89%, 0.2738 and 4.2897, respectively, higher than those of non-core population whose PPL, I and Nm were 81.37%, 0.2703 and 3.5892, respectively. However, Nei’s gene diversity index(H) and genetic differentiation coefficient(Gst) in the core population were 0.1657 and 0.1044, lower than those of non-core population whose H and Gst was 0.1662 and 0.1223. 【Conclusion】 These results showed that there were rich genetic diversity in both isolates of core and non-core regions and wide gene flow existed among these populations, but the genetic variation was mainly within the population. In contrast, the genetic diversity and the degree of genetic differentiation in the isolates of core region were relatively larger.
Minghai ZHU, Lei PI, Canwei SHU, Erxun ZHOU . AFLP Analyses of Genetic Diversity and Population Genetic Structure of Magnaporthe oryzae from South China Crop Breeding Area[J]. Chinese Journal OF Rice Science, 2017 , 31(3) : 320 -326 . DOI: 10.16819/j.1001-7216.2017.6136 320
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