Chinese Journal OF Rice Science >
Analysis of Genetic Similarity and Construction of DNA Fingerprinting for Conventional Double Cropping Early Rice Varieties in the Yangtze River Basin
Received date: 2016-08-01
Revised date: 2016-09-24
Online published: 2017-01-10
【Objective】 To provide a reference for rice variety identification and parents selection,we constructed fingerprints of conventional early rice varieties and analyzed its genetic similarity. 【Method】 Conventional early rice varieties registered at or above the provincial level since 2000 in the Yangtze River Basin were analyzed using 50 pairs of SSR primers with high polymorphism, good stability, and even distribution on 12 chromosomes of rice to construct SSR fingerprints of conventional early rice. Cluster analysis was conducted with NTSYS PC Version 2.10e software and UPGMA cluster analysis method. 【Results】 A total of 154 polymorphic bands were detected among 62 rice lines. Two to six alleles were detected for each marker with an average of 3.08. Primers polymorphism information content (PIC) value ranged from 0.03 to 0.75, with an average of 0.36. Cluster analysis with NTSYS PC Version 2.10e software showed that the genetic similarities ranged from 0.66 to 1.00. The 62 varieties could be divided into two groups as varieties in Zhejiang Province and varieties outside the province at the similarity coefficient of 0.73 with small difference between groups. 【Conclusion】 Combination with the pedigree relationship, it was revealed that the genetic background of early rice varieties in this study was relatively narrow.
Key words： early rice; pedigree; DNA fingerprinting; clustering analysis
Dongqing DAI, Huali ZHANG, Meng ZHANG, Ximing LI, Liangyong MA . Analysis of Genetic Similarity and Construction of DNA Fingerprinting for Conventional Double Cropping Early Rice Varieties in the Yangtze River Basin[J]. Chinese Journal OF Rice Science, 2017 , 31(1) : 40 -49 . DOI: 10.16819/j.1001-7216.2017.6117
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