Chinese Journal OF Rice Science >
QTL Mapping of japonica Rice Stigma Exsertion Rate
Received date: 2016-03-14
Revised date: 2016-07-19
Online published: 2017-01-10
【Objective】Stigma exsertion is one of the important traits which contribute to the efficient improrement of commercial seed production in hybrid rice. The objective of the study was to understand the genetic factors involved in the sigma exsertion.【Method】 The F2 and F2:3 populations of Da S×D50 planted in Lingshui, Hainan Province and Hangzhou, Zhejiang Province, respectively were used and the single stigma exsertion rate (PSES), double stigma exsertion rate (PDES) and total stigma exsertion rate (PES) were measured. 【Result】Correlation analysis indicated that there were significant relationships among the PSES, PDES and PES. Further, the genetic linkage map of Da S×D50 F2 population was constructed, then, 21 stigma exsertion rate QTL were located on chromosome 1,2,3,4,6,7 and 12, respectively, with the contribution rate ranging from 0.1% to 57.6%. The additive effect of most QTLs were contributed by high value parent Da S except for some QTL that with extremely low contribution rate. There were two QTLs on Chromosome 4 and Chromosome 12, qPDES4 and qPES12, both detected in F2 and F2:3 populations with contribution rate as high as 17.86% and 16.98%, respectively. The qPES3 located on Chromosome 3 with high contribution rate were detected in F2:3 population for two times. 【Conclusion】Some stigma exsertion rate QTL shared the same location with those reported by other researchers, but there were also some new QTLs which provide theoretical support for further fine-mapping, gene cloning, and breeding new japonica male sterile lines with high stigma exsertion rate by using molecular marker-assistant selection.
Wei LI, Zhonghua SHENG, Ziliang ZHU, Xiangjin WEI, Lei SHI, Yawen WU, Shaoqing TANG, Jianlong WANG, Peisong HU . QTL Mapping of japonica Rice Stigma Exsertion Rate[J]. Chinese Journal OF Rice Science, 2017 , 31(1) : 23 -30 . DOI: 10.16819/j.1001-7216.2017.6043
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