Chinese Journal OF Rice Science >
Analysis on Comparative Proteomics of Rice Grain Between Heat-tolerant and Heat-sensitive Lines Under High Night Temperature Stress at Filling Stage
Received date: 2016-06-24
Revised date: 2016-09-17
Online published: 2017-01-10
【Objective】To understand molecular mechanism of rice tolerance to high night temperature at filling stage,we identify the differentially expressed proteins (DEPs) and screen the proteins involving in regulating the heat-tolerance in rice. 【Method】The heat-tolerant rice line XN0437T and the heat-sensitive rice line XN0437S originated from a near-genetic lines were used as plant materials. Rice was pot-cultivated under traditional management. To ensure that only grain samples with uniform growth were used for proteomes analysis, rice panicle bloomed on the same date were labeled with same markers and spikelets flowering on the same date on the labeled panicle were further labeled. On the 8th day after flowering, rice plants with same label were transferred to chambers for high night temperature treatment.Rice grains with same label were harvested and total proteins of the grain samples were extracted after high temperature treatment. The 8-plex iTRAQ kits combined with the LC-MS/MS technology were used to analyze the DEPs between the heat-tolerant and heat-sensitive lines. 【Result】After protein’s databases searching and differentially expressed proteins analysis, 3130 proteins were finally identified and 36 proteins showed differential expression levels between the heat-tolerant and heat-sensitive line. For the 36 DEPs, 14 proteins (38.9%) had functional annotation, 12 (33.3%) had putative function, and 10 (33.3%) were functional unknown proteins. The 14 function-known proteins were involved in energy metabolism (five proteins), transport and metabolism (three proteins), photosynthesis (two proteins) and defense response (four proteins). 【Conclusion】High night temperature impacts the expressed patterns of the proteins involved in energy metabolism, matter transport and metabolism, photosynthesis and defense response in rice at filling stage. We suggested that up-regulated expression of the zinc finger proteins (Q67TK9 and Q10N88), and down-regulated expression of the zinc finger protein (Q5YLY5) in rice grain could enhance the heat-tolerance in rice.
Key words： rice; filling stage; high night temperature; proteomics; iTRAQ technique
Xiaoping HUANG, Hongyu ZHANG, Gang LEI, Zhimei WANG, Zhi ZHANG, Chao HE, Jianglin LIAO, Yingjin HUANG . Analysis on Comparative Proteomics of Rice Grain Between Heat-tolerant and Heat-sensitive Lines Under High Night Temperature Stress at Filling Stage[J]. Chinese Journal OF Rice Science, 2017 , 31(1) : 13 -22 . DOI: 10.16819/j.1001.7216.2017.6101
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