Chinese Journal OF Rice Science >
Molecular Cloning and Functional Analysis of NlTgo in the Rice Brown Planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
Received date: 2016-03-01
Revised date: 2016-04-21
Online published: 2016-11-10
Transcriptional regulator Tango (Tgo) plays crucial roles in insect development, including neurogenesis, hematopoiesis, sex determination and gut development. In this study, a full-length cDNA of Tango was cloned in Nilaparvata lugens. qRT-PCR and RNA interference (RNAi) were further used to analyze the expression pattern and function role, respectively. Our results showed that NlTgo contained a 2007-bp open reading frame (ORF), encoding 669 amino acid residues. Sequence alignment showed that NlTgo shared an identity of 68% with Pediculus humanus. Phylogenetic analysis suggested that NlTgo was closely related to the Tango proteins from Acyrthosiphon pisum, Pyrrhocoris apterus and Cimex lectularius. Expression profile revealed that NlTgo expression was higher in the first- and second-instar larvae than that in eggs. Furthermore, the expression level of NlTgo was higher in ovary than that in integument. Knocking down of NlTgo, in the 4th-instar nymph, was performed by double-stranded RNA (dsRNA) targeting NlTgo. It was found that the expression level of NlTgo, 4 days after injection, was significantly decreased by 77%, compared with control. Furthermore, nymphs died due to the abnormal molting, and the survival rate was only 23%, significantly lower than control group (98%). The results suggest that NlTgo is involved in the development of N.lugens and can serve as a potential target for controlling the brown planthopper.
Key words： Nilaparvata lugens; Tango; RNAi; molting; pest control
Long-fei CHEN, Pin-jun WAN, Wei-xia WANG, Qiang FU, Ting-heng ZHU . Molecular Cloning and Functional Analysis of NlTgo in the Rice Brown Planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)[J]. Chinese Journal OF Rice Science, 2016 , 30(6) : 653 -660 . DOI: 10.16819/j.1001-7216.2016.6032
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