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Research on DNA Molecular Digital Fingerprint Database Based on 48 Pairs of SSR Primers for 94 Hybrid Rice Parents in NYT 1433-2014
Received date: 2015-12-29
Revised date: 2016-03-12
Online published: 2016-11-10
It is of great significance to establish the simple molecular fingerprinting technique with high resolution for identification of the genetic polymorphism and the authenticity of different rice varieties, so as to guide rice breeding and regulate its seed market. The new standards of technical regulation on identification of the rice varieties with SSR markers, formulated by the Ministry of Agriculture of P.R. China, recommended the use of 35 control standard reference samples with different genetic characteristics for identification of rice varieties. The genetic polymorphism and specificity of 94 hybrid rice parents were compared based on the standard method. The results indicated that the tested varieties differed at least three pairs of mutated loci or the genetic differences between the parents of hybrid rice could been well distinguished. By comparing the polymorphism of the 48 recommended primers of the new standard, 46 primers showed higher polymorphism except RM176 and RM551. Thus, higher polymorphic alternative molecular markers would be identified in other loci of the same chromosome. 16 new allelic variation sites were found and could be used as supplementary of standard fingerprint database and enrich genetic variation sites information. By analyzing molecular fingerprint of 94 hybrid parental materials, 23 have specific molecular markers and can be used in authenticity analysis of hybrid combination and purity identification of hybrid seeds. According to the digital molecular fingerprint of the tested rice parent, we constructed a virtual digital molecular fingerprint database including 87 female sterile lines and 7 male parents, and specific digital molecular marker of virtual hybridized combination authenticity and rapid seed purity identification.
Key words： hybrid rice; SSR primer; digital fingerprint
LIN Yixia, WANG Zixin, LIU Huan, WANG Zheng, LIANG Manzhong, DAI Xiaojun*, CHEN Liangbi* . Research on DNA Molecular Digital Fingerprint Database Based on 48 Pairs of SSR Primers for 94 Hybrid Rice Parents in NYT 1433-2014[J]. Chinese Journal OF Rice Science, 2016 , 30(6) : 593 -602 . DOI: 10.16819/j.1001-7216.2016.6028
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