Chinese Journal OF Rice Science >
Phenotyping and Gene Cloning of a Floury Endosperm Mutant ws in Rice
Received date: 2016-03-19
Revised date: 2016-04-28
Online published: 2016-09-10
In this study, we obtained a stably inherited floury endosperm mutant ws from japonica variety cv. Dianjingyou 1 (DJY), with its 1000-grain weight, grain size, total starch content, and amylose content in mature seeds all decreased compared with wild-type, as well as the swelling power of starch. By observing the structure of mature and developing endosperm, we found that the starch granules in ws mutant were smaller and loosely packed. The WS locus was first mapped to a region near the centromere in chromosome 8 with 92 recessive individuals, and then was narrowed down to a 95 kb region by using 2025 recessive individuals. Sequence analysis revealed that the coding region of the small subunit of adenosine diphosphate glucose pyrophosphorylase (AGPS2) had a single nucleotide substitution, resulting in a change in the amino acid sequence. RT-PCR analysis showed no significant difference in the expression levels of genes encoding the AGPase subunits in the mutant endosperm, while immunoblot analysis revealed a reduced protein content of the AGPS2b. Meanwhile, the enzyme activity of AGPase in the mutant was decreased to half of the wild-type. These results showed that the mutation of OsAGPS2 caused the decreased activity of AGPase, thus affecting the starch biosynthesis in rice endosperm.
Peng-yi PAN, Jian-ping ZHU, Yun-long WANG, Yuan-yuan HAO, Yue CAI, Wen-wei ZHANG, Ling JIANG, Yi-hua WANG, Jian-min WAN . Phenotyping and Gene Cloning of a Floury Endosperm Mutant ws in Rice[J]. Chinese Journal OF Rice Science, 2016 , 30(5) : 447 -457 . DOI: 10.16819/j.1001-7216.2016.6048
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