Chinese Journal OF Rice Science >
Roles of Two Genes Involved in Histidine Biosynthetic Pathway in YeastLike Symbiont in Development of Nilaparvata lugens (Stl)
Received date: 2016-02-22
Revised date: 2016-03-04
Online published: 2016-07-10
Nilaparvata lugens is a serious phloemfeeding pest of rice in China. Based on genome and transcriptome data of N.lugens and yeastlike symbiont (YLS, also named Entomomyces delphacidicola str. NLU), the major biosynthesis pathways for amino acids in N.lugens were constructed. In this study, we cloned two genes, EdeHis2 and EdeHis6, which catalyze critical steps in histidine biosynthesis pathway, and revealed the negative effects of doublestranded RNA (dsRNA) on the growth, development and survival rate of N.lugens. Homology searches and phylogenetic analysis showed that EdeHis2 and EdeHis6 origin from YLS genome, share high similarities with that of Metarhizium acridum and formed a clad in the phylogenetic tree, whereas no His2 or His6like genes was found in N.lugens genome. Temporal expression profiles of EdeHis2 and EdeHis6 showed that both genes were ubiquitously but unevenly expressed among the different life stages, and the spatial expression pattern showed they have higher expression levels in the fat body rather than head, leg, integument and midgut. Furthermore, no target products were amplified in head and wing genomic DNA of N. lugens, rather than that in abdomen genomic DNA. At two, four and six days after dsEdeHis2 or dsEdeHis6 injection, the mRNA abundance of target genes was decreased by 45%-60% (EdeHis2)or 27%-55% (EdeHis6), comparing with blank control.Downregulation of EdeHis2 or dsEdeHis6 slightly increased the mortality by 8.3% or 9.2%, and delayed the nymphal duration of male and female by 0.43 and 065 day and 0.33 and 0.36 day, respectively. Moreover, both male (11%) and female adult (13%) showed wing deformation after injection of dsEdeHis6, higher than that in the blank control. In conclusion, EdeHis2 and EdeHis6 that origin from YLS were involved in histidine biosynthesis pathway, contributed to the survivor, development and wing formation of N. lugens.
Key words： Nilaparvata lugens; yeastlike symbiont; histidine; His2; His6; RNAi
TANG Yaohua1,2, WAN Pinjun2, HAO Peiying1, FU Qiang2, *, YU Xiaoping1, * . Roles of Two Genes Involved in Histidine Biosynthetic Pathway in YeastLike Symbiont in Development of Nilaparvata lugens (Stl)[J]. Chinese Journal OF Rice Science, 2016 , 30(4) : 406 -416 . DOI: 10.16819/j.1001-7216.2016.6026
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