Chinese Journal OF Rice Science >
Identification and Gene Fine Mapping of a Brittle Culm 16 (bc16) Mutant in Rice
Received date: 2015-12-14
Revised date: 2016-02-26
Online published: 2016-07-10
Brittle culm mutants are one of the important materials to study plant mechanical strength. A brittle culm mutant named as bc16 (brittle culm 16) was isolated from Nipponbare through EMS (Ethyl Methane Sulphonate) treatment. Compared with wild type, bc16 featured fragile and easily broken stems,leaves,roots, lower plant height and filled grains per panicle, shorter panicle length and main root length. The components analysis of stem cell wall showed that the cellulose content in bc16 mutant was sharply decreased, while hemicellulose content was evidently increased and no significant difference had been found in the lignin content between bc16 mutant and wild type. The cell shape and arrangement of parenchyma cells in bc16mutant became irregular and disordered. Moreover, the parenchyma cell walls and the secondary cell walls of sclerenchyma cells in bc16 mutant under the epidermis layer were thinner than those in wild type. The results of genetic analysis revealed that the mutant trait was controlled by a single recessive gene, which was mapped in a 66.6kb region between InDel (insertiondeletion) markers 2F and 2H on chromosome 2 with 7 candidate genes. Among these genes, Os02g0738900, which is allelic to BC3 gene, was specially focused on. There was a T to A substitution at the very end of the 13th intron of Os02g0738900 which was at the 5113th base location from initiation codon by sequencing, which led an splicing forward mutation in the process of transcription, and six nucleotides were cut into mRNA as a result, which finally cause the stop of translation. Additionally, in the mutant, there were significant decrease in the expression of bc16 gene in roots, culms and leaves by realtime PCR method. The bc16 might affect the synthesis of sclerenchyma secondary cell wall and parenchyma primary cells wall, which could affect the mechanical strength of rice stem.
Key words： rice; brittle culm; gene mapping; cell wall synthesis
SHU Yazhou, ZENG Dongdong, QIN Ran, JIN Xiaoli, ZHENG Xi, SHI Chunhai* . Identification and Gene Fine Mapping of a Brittle Culm 16 (bc16) Mutant in Rice[J]. Chinese Journal OF Rice Science, 2016 , 30(4) : 345 -355 . DOI: 10.16819/j.1001-7216.2016.5184
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