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Expression Characteristics and Sequence Variation Analysis of Glutamine Synthetase Gene in Grain of japonica Rice with Transgressive Variation
Received date: 2015-10-19
Revised date: 2015-12-23
Online published: 2016-05-10
The transgressive variants derived from rice varieties with significant difference in grain protein content were used to investigate the expression level and structure of glutamine synthetase gene(GS), activity of glutamine synthetase and protein accumulation characteristics during grain filling. The results showed that transgressive variants could be obtained through successive directive selection of grain protein content, because the grain protein content was closely related to corresponding genotype. During grain filling, the activity of glutamine synthetase followed a single-peak curve, highly correlated with grain protein content and significantly differed between parents and progenies. GS1.3 and GS2 genes, in transgressive variants and their parents with different protein content, exhibited similar transcription trend during grain filling, that is, the transcription level increased 15-20 days before heading and then decreased gradually. Moreover, the grain protein content was closely related to GS1.3 and GS2 expression levels. The varieties with higher GS transcription level showed higher protein content compared with their parents. In addition, although GS1.3 and GS2 gene sequences showed high conservation, the gene sequence and protein sequence of GS1.3 and GS2 were not completely identical in different varieties, and there are some single nuclear polymorphisms. Random base variation as well as changes in codon and amino acids might occur because inter-variety sexual hybridization causes base substitution during segregation and stability.
Key words： japonica rice; transgressive variant; grain; GS gene expression; sequence variation
Zhen-hua XU, Ying QU, Hai-ying LIU, Li-nan ZHU, Zhong-chen ZHANG, Zheng-xun JIN . Expression Characteristics and Sequence Variation Analysis of Glutamine Synthetase Gene in Grain of japonica Rice with Transgressive Variation[J]. Chinese Journal OF Rice Science, 2016 , 30(3) : 304 -312 . DOI: 10.16819/j.1001-7216.2016.5150
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