Identification and analysis of the variations of rice blast resistance gene sequence is the basis to dig into the genetic resources of the rice blast resistance. In this paper, 609 bases in the second code functional region of the rice blast resistance gene Pita were analyzed using 218 rice landraces from 56 counties of Yunnan Province. A total of 18 nucleotide variations were found in the sequence and the ORFs of 218 rice accessions were grouped into 46 haplotypes and 28 proteins types. Pita-H1 (17.89%), Pita-H2(29.82%) and Pita-H6(20.18%) were high-frequency types in Yunnan, while other types were at low frequency (0.46%-3.67%). So the advantage haplotypes in Yunnan were Pita-H1, Pita-H2 and Pita-H6 (totally 67.89%). A total of 61 rice landraces carrying the resistance gene Pita, the base functional site 2752 was G with the rate of 27.98%. The sequence of Pita in 39 resistance varieties was identical with the sequence obtained from the Vietnamese resistance variety Tetep. The distribution of resistance gene Pita in rice subspecies or ecotypes was unbalanced. The distribution frequency of indica was 35.06%, japonica 24.11%, rice 31.03%, upland rice 21.92%, no-glutinous rice 30.77%, glutinous rice 18.37%. The distribution of blast resistance gene Pita in Yunnan Province featured wide distribution and small concentration. Pita gene was detected in 36 counties of Yunnan Province. The distribution frequency in southern Yunnan was higher than other area. Moreover, some counties were of high distribution frequency. The resistance gene Pita was not detected in seven rice varieties growing over 2200 m elevation.