Chinese Journal OF Rice Science >
Effects of Bt Rice with cry1C and cry2A on the Ecological Generation Fitness of Rice Brown Planthoppers(Nilaparvata lugens) and Whitebacked Planthoppers (Sogatella furcifera) at Various Nitrogen Rates
Received date: 2015-06-01
Revised date: 2015-12-18
Online published: 2016-03-10
In order to evaluate the effects of Bt rice on the generation fitness of non-target pest at different nitrogen rates, as wall as the potential impact of Bt rice and nitrogen fertilizer levels on non-target pest, the brown plantthopper (BPH) (Nilaparvata lugens) and the whitebacked planthopper (WBPH) (Sogatella furcifera), were successively cultured for four generations on Bt rice, T1C-19 with cry1C and T2A-1 with cry2A , and their parental rice Minghui 63, at nitrogen rates of 0, 100 and 250 kg/hm2. The results indicated that the ecological fitness of the BPH and WBPH was increased with the increasing nitrogen fertilizer level, including shortened nymph developmental duration, enhanced fecundity, prolonged longevity, increased nymph survival rate, female weight, egg hatchability and population growth rate. There were significant differences in rice planthopper nymphal development duration, body weight of female adult, fecundity and population growth rate for four successive generations under different nitrogen rates. Transgenic Bt rice, T1C-19 and T2A-1, had no significant effect on the fitness of BPH and WBPH at the same generations and nitrogen rates, except the BPH fecundity. BPH fecundity on transgenic Bt rice T1C-19 at the nitrogen levels of 100 kg/hm2 and 250 kg/hm2(N) was lower compared with the transgenic Bt rice T2A-1 and its parent Minghui 63 from the first generation to the third generation, but no significant difference was found among varieties at the fourth generation at three nitrogen rates. No significant difference was found on the fitness of BPH and WBPH with the generation increase, between non-transgenic Bt rice and transgenic Bt rice. Therefore, the present study demonstrated that nitrogen fertilizer levels exerted a significant influence on the ecological fitness of the rice planthoppers with the generation increase, while transgenic Bt rice didn't.
Kai LIU, Ya-jun YANG, Jun-ce TIAN, Yan-hui LU, Hong-xing XU, Xu-song ZHENG, Zhong-xian LV . Effects of Bt Rice with cry1C and cry2A on the Ecological Generation Fitness of Rice Brown Planthoppers(Nilaparvata lugens) and Whitebacked Planthoppers (Sogatella furcifera) at Various Nitrogen Rates[J]. Chinese Journal OF Rice Science, 2016 , 30(2) : 200 -209 . DOI: 10.16819/j.1001-7216.2016.5090
| [1] | 程家安, 祝增荣. 2005年长江流域稻区褐飞虱暴发成灾原因分析. 植物保护, 2006, 32: 1-4. |
| [1] | Chen J A, Zhu Z R.Analysis on the key factors causing the outbreak of brown planthopper in Yangtze Area,China in 2005.Plant Prot, 2006. 32:1-4.(in Chinese with English abstract) |
| [2] | 林拥军, 华红霞, 何予卿, 等. 水稻褐飞虱综合治理研究与示范——农业公益性行业专项“水稻褐飞虱综合防控技术研究”进展. 应用昆虫学报, 2011, 48(5): 1194-1201. |
| [2] | Lin Y J, Hua H X, He Y Q, et al.Progress in research on the integrated management of the brown planthopper, Nilaparvata lugens (Stål) in China.Chin J Appl Entomol, 2011, 48(5): 1194-1201.(in Chinese with English abstract) |
| [3] | Gallagher K D, Kenmore P E, Sogawa K.Judicial use of insecticides deter planthopper outbreaks and extend the role of resistant varieties in Southeast Asian rice//DennoR F, Perfect T J. Plant hoppers: Their Ecology and Management. London: Chapman Hall, 1994: 559-614. |
| [4] | 吕仲贤, 俞晓平, Heong K L, 等. 氮肥对水稻叶冠层捕食性天敌种群及其自然控制能力的影响. 植物保护学报,2006, 33(3): 225-229. |
| [4] | Lu Z X, Yu X P, Heong K L, et al.Dynamics of predators in rice canopy and capacity of natural control on insect pests in paddy fields with different nitrogen regimes.Acta Phytophyl Sin, 2006, 33(3):225-229.(in Chinese with English abstract) |
| [5] | Sogawa K.Windborn displacements of rice planthoppers related to the seasonal weather patterns in Kyushu district.Bull Kyushu Nat Agri Exper Stat, 1995, 28(4): 219-278. |
| [6] | Conway G R, Barbier E B.After the Green Revolution: Sustainable Agriculture for Development. London: Earthscan Publications Ltd, 1990. |
| [7] | Sinclair T R.Historical changes in harvest index and crop nitrogen accumulation.Crop Sci, 1998, 38:638-643. |
| [8] | 邓伟, 胡兰香, 陈红萍, 等. 水稻抗白背飞虱研究进展. 江西农业学报,2012, 24(1): 91-97. |
| [8] | Deng W, Hu L X, Chen H P, et al.Research progress in resistance of rice to Sogatella furcifera.Acta Agric Jiangxi, 2012, 24(1): 91-97.(in Chinese with English abstract) |
| [9] | Zhou G H, Wen J J, Cai D J,et al.Southern rice black-streak dwarf virus: A new proposed Fijivirus species in the family Reoviridae.Chin Sci Bull, 2008, 53: 3677-3685. |
| [10] | Li Y Z, Cao Y, Zhou Q, et al.The efficiency of southern rice black-streaked dwarf virus transmission by the vector Sogatella furcifera to different host plant species.J Integ Agric, 2012, 11:621-627. |
| [11] | Tu J, Zhang G, Datta K,et al.Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis δ-endotoxin.Nat Biotechnol, 2000, 18(10):1101-1104. |
| [12] | Datta K, Vasquez A, Tu J, et al.Constitutive and tissue-specific differential expression of the cryIA ( b ) gene in transgenic rice plants conferring resistance to rice insect pests.Theoret Appl Genet, 1998, 97: 20-30. |
| [13] | Shu Q Y, Ye G Y, Cui H R, et al.Transgenic rice plants with a synthetic cry1Ab gene from Bacillus thuringiensis were highly resistant to eight lepidopteran rice pest species.Mol Breeding, 2000, 6(4): 433-439. |
| [14] | Ye G Y, Shu Q Y, Yao H W,et al.Field resistance evaluation of transgenic containing a synthetic cry 1Ab gene from Bacillus thuringiensis Berliner to two stem borers.J Econom Entomol, 2001, 94(1): 271-276. |
| [15] | Ye G Y, Tu J, Hu C, et al.Transgenic IR72 with fused Bt gene cry 1Ab/ cry 1Ac from Bacillus thuringiensis is resistant against four lepidopteran species under field conditions.Plant Biotechnol, 2001, 18(2): 125-133. |
| [16] | Ye G Y, Yao H W, Shu Q R, et al.High levels of stable resistance in transgenic rice with a synthetic cry 1Ab gene from Bacillus thuringiensis Berliner to rice leaffolder, Cnaphalocrocis medinalis (Guenée) under field conditions.Crop Prot, 2003, 22(1):171-178. |
| [17] | Qiu J.Agriculture: Is China ready for GM rice?Nature (Lond.), 2008, 455(7215): 850-852. |
| [18] | Akhtar Z R, Tian J C, Chen M,et al.Impacts of six Bt rice lines on nontarget rice feeding thrips under laboratory and field conditions.Environ Entomol, 2010, 39(2): 715-726. |
| [19] | 傅强, 王锋, 李冬虎, 等. 转基因抗虫水稻MSA和MSB对非靶标害虫褐飞虱和白背飞虱的影响. 昆虫学报, 2003,46(6):697-704. |
| [19] | Fu Q, Wang F, Li D H, et al.Effects of insect-resistant transgenic rice lines MSA and MSB on non-target pests Nilaparvata lugens and Sogatellafucifera.Acta Entomol Sin, 2003, 46(6):697-704.(in Chinese with English abstract) |
| [20] | 谭红, 叶恭银, 胡萃, 等,转 cry1Ab 基因抗虫籼稻对非靶标害虫白背飞虱发育与繁殖的影响. 植物保护学报, 2006,33(3):251-256. |
| [20] | Tan H, Ye G Y, Hu C, et al.Effects of transgen icindica rice expressing a gene of cry1Ab with insect resistance on the development and reproduction of nontarget pest, Sogatella furcifera (Homoptera: Delphacidae).Acta Phytophyl Sin, 2006, 33(3): 251-256.(in Chinese with English abstract) |
| [21] | Gao M Q, Hou S P,Chen X X, et al.Multi-generation effects of Bt rice on Anagrus nilaparvatae, a parasitoid of the nontarget pest Nilapavarta lugens.Economic Entomol, 2010, 39(6): 2039-2044. |
| [22] | 姜永厚, 傅强, 程家安, 等. 转Bt基因水稻对二化螟绒茧蜂生物学特性的影响.昆虫学报, 2004, 47(1): 124-129. |
| [22] | Jiang Y H, Fu Q, Cheng J A, et al.Effects of transgenic Bt rice on the biological characteristics of Apanteles chilonis (Munakata) (Hymenoptera: Braconidae).Acta Entomol Sin. 2004, 47(1): 124-129. (in Chinese with English abstract) |
| [23] | 白耀宇, 蒋明星, 程家安. 转Bt基因水稻对两种弹尾虫及尖钩宽黾蝽捕食作用的影响. 昆虫学报,2005, 48(1):42-47. |
| [23] | Bai Y Y, Jiang M X, Cheng J A.Impacts of transgenic cry1Ab rice on two collembolan species and predation of Microvelia horvathi (Hemiptera: Veliidae).Acta Entomol Sin, 2005, 48(1): 42-47.(in Chinese with English abstract) |
| [24] | Bai Y Y, Jing M X, Cheng J A.Effects of transgenic cry1Ab rice pollen on fitness of Propylea japonica (Thunberg).J Pest Sci, 2005, 78: 123-128. |
| [25] | Bai Y Y, Jing M X, Cheng J A,et al.Effects of Cry1Ab toxin on Propylea japonica (Thunberg)(Coleoptera: Coccinellidae) through its prey, Nilaparvata lugens (Stål) (Homoptera: Delphacidae), feeding on transgenic Bt rice.Entomol Soc Amer, 2006, 35(4):1130-1136. |
| [26] | 刘志诚, 叶恭银, 胡萃, 等. 转 Cry1Ab 基因水稻对拟水狼蛛捕食作用间接影响的评价. 中国水稻科学, 2003, 17(2): 175-178. |
| [26] | Liu Z C, Ye G Y, Hu C, et al.Indirect impact assessment of transgenic rice with cry1Ab gene on predations by the wolf spider, Pirata subpiraticus.Chin J Rice Sci, 2003, 17(2): 175-178.(in Chinese with English abstract) |
| [27] | Tian J C, Liu Z C, Chen M,et al.Laboratory and field assessments of prey-mediated effects of transgenic Bt rice on Ummeliata insecticeps (Araneida:Linyphiidae).Econom Entomol, 2010, 39(4): 1369-1377. |
| [28] | Bai Y Y, Yan R H, Ke X, et al.Effects of transgenic Bt rice on growth, reproduction, and superoxide dismutase activity of Folsomia candida (Collembola: Isotomidae) in Laboratory studies.J Econom Entomol, 2011, 104(6):1892-1899. |
| [29] | 吕仲贤, Villareal S, 俞晓平, 等. 氮肥对稻株含水量和伤流液的影响及其与对褐飞虱为害耐性的关系. 中国水稻科学, 2004, 18(2): 161-166. |
| [29] | Lu Z X, Villareal S, Yu X P, et al.Effect of nitrogen on water content, sap flow of rice plants in association with tolerance to brown planthopper, Nilaparvata lugens.Chin J Rice Sci, 2004, 18(2):161-166.(in Chinese with English abstract) |
| [30] | 冯涛, 杨京平, 施宏鑫,等. 高肥力稻田不同施氮水平下的氮肥效应和几种氮肥利用率的研究. 浙江大学学报:农业与生命科学版, 2006, 32(1): 60-64. |
| [30] | Feng T, Yang J P, Shi H X, et al.Effect of N fertilizer and N use efficiency under different N levels of application in high-fertility paddy field. J Zhejiang Univ:(Agric Life Sci), 2006, 32(1): 60-64.(in Chinese with English abstract) |
| [31] | Simpson S J, Simpson C L.The mechanisms of nutritional compensation by phytophagous insects//Bernays E A. Insect-plant interactions, Vol.Ⅱ. New York: CPC Press,Inc, 1990: 111-160. |
| [32] | Lu Z X, Heong K L, Yu X P, et al.Effects of plant nitrogen on ecological fitness of the brown planthopper, Nilaparvata lugens, in rice.J Asia-Pac Entomol, 2004, 7(1): 97-104. |
| [33] | 吕仲贤, 俞晓平, Heong K L, 等. 氮肥对植食性昆虫的影响及其对水稻主要害虫种群的诱导. 中国水稻科学, 2006, 20(6): 649-656. |
| [33] | Lu Z X, Yu X P, Heong K L, et al.Nitrogen fertilizer affects herbivores and stimulates the populations of major insect pest of rice .Chin J Rice Sci, 2006, 20(6): 649-656.(in Chinese with English abstract) |
| [34] | 吕仲贤, 俞晓平, Heong K L, 等. 稻田氮肥施用量对黑肩绿盲蝽捕食功能的影响. 昆虫学报, 2005, 48(1): 48-56. |
| [34] | Lu Z X, Yu X P, Heong K L, et al.Effects of nitrogenous fertilization in rice fields on the predatory function of Cyrtorhinus lividipennis reuter to Nilaparvata lugens Stål.Acta Entomol Sin, 2005, 48(1):48-56.(in Chinese with English abstract) |
| [35] | Lu Z X, Yu X P, Heong K L, et al.Effects of nitrogen nutrient on the behavior of feeding and oviposition of the brown planthopper, Nilaparvata lugens, on IR64. J Zhejiang Univ:Agric Life Sci, 2005, 31(1): 62-70. |
| [36] | 陈洋, 田俊策, 彭于发, 等. 转 Cry1Ab/vip3H 基因水稻对非靶标害虫褐飞虱连续多代生长发育与繁殖的影响. 中国生物防治学报, 2011, 27(4): 490-497. |
| [36] | Chen Y, Tian J C, Peng Y F, et al.Multi-generation effects of transgenic cry1Ab/vip3H rice G6H1 on development and reproduction of the non-target pest, Nilaparvata lugens (Stål) .Chin J Biol Cont, 2011, 27(4): 490-497.(in Chinese with English abstract) |
| [37] | Chen M, Ye G Y, Liu Z C, et al.Field assessment of the effects of transgenic rice expressing a fused gene of cry1Ab and cry1Ac from Bacillus thuringiensis Berliner on nontarget planthoppers and leafhoppers populations.Environ Entomol, 2006, 35: 127-134. |
| [38] | 陈茂, 叶恭银, 胡萃, 等. Bt水稻对飞虱和叶蝉及其卵寄生蜂扩散规律的影响. 浙江大学学报:农业与生命科学版, 2003, 29(1): 29-33. |
| [38] | Chen M, Ye G Y, Hu C, et al.Effect of transgenic Bt rice on dispersal of planthoppers and leafhoppers as well as their egg parasitic wasps . J Zhejiang Agric Univ (Agric Life Sci), 2003, 29(1): 29-33.(in Chinese with English abstract) |
| [39] | Chen M, Liu Z C,Ye G Y, et al.Impacts of transgenic Cry1Ab rice on non-target planthoppers and their main predator Cyrtorhinus lividipennis (Hemiptera: Miridae)-A case study of the compatibility of Bt rice with biological control.Biol Cont, 2007, 42: 242. |
| [40] | Xu X L, Han Y,Hua H X, et al.Field evaluation of effects of transgenic cry1Ab/cry1Ac,cry1C and cry2A rice on Cnaphalocrocis medinalis and its arthropod predators.Sci China Life Sci, 2011, 54:1019-1028. |
| [41] | Han Y, Xu X L, Ma W H, et al.The influence of transgenic cry1Ab/cry1Ac, cry1C and cry2A rice on non-target planthoppers and their main predators under field conditions.Agric Sci China, 2011, 10(11): 1739-1747. |
| [42] | 刘志诚, 叶恭银, 胡萃, 等. 转 cry 1 Ab / cry 1 Ac 基因籼稻对稻田节肢动物群落影响. 昆虫学报, 2003, 46(4): 454-465. |
| [42] | Liu Z C, Ye G Y, Hu C, et al.Impact of transgenic indica rice with a fused gene of cry1Ab/cry1Ac on the rice paddy arthropod community.Acta Entomol Sin, 2003, 46(4): 454-465.(in Chinese with English abstract) |
| [43] | 刘志诚, 叶恭银, 胡萃. 抗虫转基因水稻和化学杀虫剂对稻田节肢动物群落的影响. 应用生态学报, 2004, 15(12): 2309-2314. |
| [43] | Liu Z C, Ye G Y, Hu C.Effects of Bacillus thuringiensis transgenic rice and chemical insecticides on arthropod communities in paddy fields.Chin J Appl Ecol, 2004, 15(12): 2309-2314.(in Chinese with English abstract) |
| [44] | 刘凯, 杨亚军, 吕仲贤, 等. 转 cry1C 和 cry2A 基因Bt水稻对白背飞虱生长、发育及繁殖的继代影响. 浙江农业学报, 2014, 26(3): 730-735. |
| [44] | Liu K, Yang Y J, Lu Z X, et al.Multi-generation effect of Bt rice with cry1C and cry2A on survival, development and reproduction of non-target pest Sogatella furcifera(Horvath).Acta Agric Zhejiangensis, 2014, 26(3): 730-735.(in Chinese with English abstract) |
| [45] | 刘雨芳, 贺玲, 汪琼, 等. 转 cry1Ac/sck 基因抗虫水稻对稻田主要非靶标害虫的田间影响评价. 中国农业科学, 2007, 40(6): 1181-1189. |
| [45] | Liu Y F, He L, Wang Q, et al.Effects of and ecological safety insect-resistant Cry1Ac/sck transgenic rice on key non-target pests in paddy fields .Sci Agric Sin, 2007, 40(6):1181-1189.(in Chinese with English abstract) |
| [46] | Timmons A M, Charters Y M, Crawford J W,et al.Risks from transgenic crops.Nature, 1996, 380(6574): 487. |
/
| 〈 |
|
〉 |