Chinese Journal OF Rice Science >
Construction and Screening of an Irradiation-induced Mutant Library of indica Rice 93-11
Received date: 2015-08-18
Revised date: 2015-10-27
Online published: 2016-01-10
Mutant libraries are powerful tools for plant germplasm creation and functional analysis of genome. Using 93-11 (O. sativa ssp. indica cv. 93-11), an elite variety with complete genome sequences available, we established an irradiation-induced mutant library containing 3617 lines. Three hundred and eight mutant lines were obtained from M2 and M3 generations and classfied into 15 types of mutation, including plant height, tiller number, plant architecture, inflorescence architecture, spikelet architecture, fertility, leaf color, leaf shape, heading date, and bentazon resistance. The mutation frequencies ranged from 0.03% to 2.74%. Using reverse genetics approach, we found that CYP704B2 is the causal gene for two male sterile mutants and CYP81A6 is responsible for two bentazon-lethal mutations. For the two male sterile mutants, nucleotide substitution and deletion were detected in the third and fourth exons of CYP704B2, respectively. For the two bentazon-lethal mutants, a 7-bp and a 23-bp deletion were detected in the first and second exons of CYP81A6, respectively. Genetic studies revealed that wild-type plants and mutant plants segregated at 3:1 ratio, suggesting that these mutant traits were controlled by single recessive genes. Our study showed that this mutant library is a useful resource for rice genome functional analysis and rice genetic improvement.
Key words： indica; mutagenesis by irradiation; mutant library; male sterility; bentazon-lethal
Tuan LONG, Bao-guang AN, Xin-peng LI, Wei ZHANG, Jing-lin LI, Yao-hua YANG, Xiang ZENG, Yong-zhong WU, Pei-jin HUANG . Construction and Screening of an Irradiation-induced Mutant Library of indica Rice 93-11[J]. Chinese Journal OF Rice Science, 2016 , 30(1) : 44 -52 . DOI: 10.16819/j.1001-7216.2016.5126
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