Chinese Journal OF Rice Science >
Sequence Variation of Pi-d3 for Rice Blast Resistance in Yunnan Rice Landrace
Received date: 2015-06-13
Revised date: 2015-07-30
Online published: 2016-01-10
The sequence variations of Pi-d3 for rice blast resistance in 80 Yunnan rice landraces were analyzed by resequencing open reading frame (ORF). Compared with Pi-d3Digu ORF, a total of 39 nucleotide variations were found in 2775-bp ORFs of 80 rice accessions with the average mutation rate of 1.41%. The WTBXPi-d3ORF of 80 rice accessions was divided into 37 haplotypes based on the nucleotide variations. H8(28.8%), H4(11.3%) and H23(5.0%) are high-frequency types, while other types occurred at low frequency. The results showed that the WTBXPi-d3 in Yunnan rice landraces was rich in genetic variations and haplotypes,but low frequency. A total of 28 amino acids variations were found in 33 translation proteins of 37 haplotypes. 18 haplotypes with 32 rice landraces including 12 indica accessions possessde the Pi-d3 pseudogenes. The frequency of pseudogene was high. It was possible due to that the Pi-d3 pseudogene permeated to indica. Terminators were newly founded at position 32 and 467 of protein except the published terminator at position 737. Pi-d3 differed in both type and frequency of haplotypes between the subspecies of indica and japonica, the ecotypes of lowland and upland rice, no-waxy and waxy rice. It indicated that genetic differentiation occurred between subspecies or ecotypes. Geographical distribution showed that 37 haplotypes spread over a large area while concentrated in small areas. Puer, Banna and Lincang were the central area with abundant haplotypes, and based on this scale, the types of haplotypes decreased outward progressively.
Key words： Yunnan rice landraces; rice blast; Pi-d3; single nucleotide polymorphism
Yi YANG, Yi-ding SUN, Ji-qiong MA, Yan-yan WANG, Ming-hui XU . Sequence Variation of Pi-d3 for Rice Blast Resistance in Yunnan Rice Landrace[J]. Chinese Journal OF Rice Science, 2016 , 30(1) : 17 -26 . DOI: 10.16819/j.1001-7216.2016.5092
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