Chinese Journal OF Rice Science >
Genetic Analysis and Gene Mapping of a Yellow-Green Leaf Mutant djyg in Rice
*Corresponding author：E-mail:wang.620@osu.edu
Received date: 2015-06-18
Revised date: 2015-07-09
Online published: 2015-11-10
A spontaneous yellow-green mutant was obtained from the japonica rice cultivar Dongjin in the field, designated as djyg. The mutant showed obvious yellow-green leaf phenotype at the seedling stage but the chlorotic leaves returned to green after the heading stage. Consistent with the phenotype, the chlorophyll b content of the mutant was reduced by 53%, 62% and 36% at the seedling, tillering and heading stages, respectively, compared with that of the wild type (WT). At the tillering stage, we observed that the granas and thylakoid stacks displayed a mess and loose structure, as well as less density in the mutant djyg compared with the WT under a transmission electron microscopy. The transcriptional levels of PORA, Cab1R, PsbA were also decreased in the mutant djyg. Genetic analysis and map-based cloning demonstrated that the phenotype of the mutant was controlled by a major recessive nuclear gene, which is an allele of chlorophyll synthase YGL1. The mutant djyg can be used as a phenotypic marker in rice breeding program since the main agronomic traits of the mutant djyg are normal compared with those of the WT.
Key words： Oryza sativa L.; yellow-green leaf; mutant; map-based cloning
Zhi-qiang LI, Dan ZHU, Zhi-long WANG, Bo DING, Guo-liang WANG . Genetic Analysis and Gene Mapping of a Yellow-Green Leaf Mutant djyg in Rice[J]. Chinese Journal OF Rice Science, 2015 , 29(6) : 601 -609 . DOI: 10.3969/j.issn.1001G7216.2015.06.006
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