Orginal Article

Gene Mapping of a Narrow and Rolled Leaf Mutant Nrl3(t) in Rice

Expand
  • 1Rice Research Institute/Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Chengdu 611130, ChinaNational Center for Rice Improvement,China National Rice Research Institute, Hangzhou, 310006, China
    2National Center for Rice Improvement,China National Rice Research Institute, Hangzhou, 310006, China
    3Zhejiang National Rice High-Tech Plants Industry Limited Company, Hangzhou 311401, China

*Corresponding author:E-mail:wuxjsau@126.com,caolycgf@mail.hz.zj.cn

Received date: 2015-02-09

  Revised date: 2015-09-06

  Online published: 2015-11-10

Abstract

Nrl3(t) was a novel narrow and rolled leaf mutant from the indica restorer line Zhonghui 8015 after exposure to 60Co-γ ray. Nrl3(t) exhibited significant adaxially-rolled leaves, reduced plant height, shortened panicle length, reduced grain length, grain width and thousand-grain weight, as well as the seed-setting rate. Cytological analysis showed that the rolled leaf phenotype was due to the reduced number of vascular bundleand the changed size of bulliform cells. In an initial mapping with F2 individuals derived from a cross between Nrl3(t) and japonica 02428, the narrow and rolled leaf gene Nrl3(t) was eventually mapped to a 70.3 kb region on the long arm of chromosome 2 between two newly developed sequence tagged site markers C9 and C10E. These results would lay a good foundation for molecular cloning and functional analysis of Nrl3(t).

Cite this article

Xiao-hui ZHANG, Ya-zhi QIN, Ying-xin ZHANG, Xiao-deng ZHAN, Zhen-hua ZHANG, Xi-hong SHEN, Shi-hua CHENG, Li-yong CAO, Xian-jun WU . Gene Mapping of a Narrow and Rolled Leaf Mutant Nrl3(t) in Rice[J]. Chinese Journal OF Rice Science, 2015 , 29(6) : 595 -600 . DOI: 10.3969/j.issn.1001G7216.2015.06.005

References

[1] 陈温福, 徐正进, 张龙步.水稻理想株型的研究.沈阳农业大学学报, 1989, 20(4):417-420.
[2] 袁隆平. 杂交水稻超高产育种.杂交水稻, 1997, 12(6):1-3.
[3] Xiang J J, Zhang G H, Qian Q, et al.SEMI-ROLLED LEAF1 encodes a putative glycosylphosphatidylinositol-anchored protein and modulates rice leaf rolling by regulating the formation of bulliform cells.Plant Physiol, 2012, 159(4): 1488-1500.
[4] Li L, Shi Z Y, Li L, et al.Overexpression of ACL1 (abaxially curled leaf 1) increased bulliform cells and induced abaxial curling of leaf blades in rice.Mol Plant, 2010, 3(5): 807-817.
[5] Zou L P, Sun X H, Zhang Z G, et al.Leaf rolling controlled by the homeodomain leucine zipper class IV gene Roc5 in rice.Plant Physiol, 2011, 156(3): 1589-1602.
[6] Hibara K,Obara M,Hayashida E, et al.The ADAXIALIZED LEAF1 gene functions in leaf and embryonic pattern formation in rice.Devel Biol,2009,334(2):345-354.
[7] Fang L K, Zhao F M, Cong Y F, et al.Rolling-leaf14 is a 2OG-Fe (II) oxygenase family protein that modulates rice leaf rolling by affecting secondary cell wall formation in leaves.Plant Biotechnol J, 2012, 10(5): 524-532.
[8] Li M, Xiong G Y, Li R, et al.Rice cellulose synthase-like D4 is essential for normal cell-wall biosynthesis and plant growth.Plant J, 2009, 60(6): 1055-1069.
[9] Wu C, Fu Y P, Hu G H, et al.Isolation and characterization of a rice mutant with narrow and rolled leaves.Planta, 2010, 232(2): 313-324.
[10] 朱丽, 胡江, 颜美仙, 等. 水稻卷窄叶突变相关基因OsCSLD4 的RNAi研究及表达分析. 核农学报, 2010, 24(5): 873-880.
[11] Hu J, Zhu L, Zeng D L, et al.Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice.Plant Mol Biol, 2010, 73(3): 283-292.
[12] Yoshikawa T, Eiguchi M, Hibara K I, et al.Rice SLENDER LEAF 1 gene encodes cellulose synthase-like D4 and is specifically expressed in M-phase cells to regulate cell proliferation.J Exper Bot, 2013, 64(7): 2049-2061.
[13] Yang C H, Li D Y, Liu X, et al.OsMYB103L, an R2R3-MYB transcription factor, influences leaf rolling and mechanical strength in rice (Oryza sativa L.).BMC Plant Biol, 2014, 14: 158.
[14] Luan W J, Liu Y Q, Zhang F G, et al.OsCD1 encodes a putative member of the cellulose synthase-like D sub-family and is essential for rice plant architecture and growth.Plant Biotechnol J, 2011, 9(4): 513-524.
[15] Shi Z Y, Wang J, Wan X S, et al.Over-expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit.Planta, 2007, 226(1): 99-108.
[16] Zhang G H, Xu Q, Zhu X D, et al.SHALLOT-LIKE1 is a KANADI transcription factor that modulates rice leaf rolling by regulating leaf abaxial cell development.Plant Cell, 2009, 21(3): 719-735.
[17] Wu R H, Li S B, He S, et al.CFL1, a WW domain protein, regulates cuticle development by modulating the function of HDG1, a class IV homeodomain transcription factor, in rice and Arabidopsis.Plant Cell, 2011, 23(9): 3392-3411.
[18] Fujino K,Matsuda Y, Ozawa K, et al.NARROW LEAF 7 controls leaf shape mediated by auxin in rice.Mol Genet Genom, 2008, 279(5): 499-507.
[19] Cho S H, Yoo S C, Zhang H T, et al.The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A (OsWOX3A) and function in leaf, spikelet, tiller and lateral root development.New Phytol, 2013, 198(4): 1071-1084.
[20] Qi J, Qian Q, Bu Q Y, et al.Mutation of the rice narrow leaf1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport.Plant Physiol, 2008, 147(4): 1947-1959.
[21] Chen M L,Luo J, Shao G N, et al.Fine mapping of a major QTL for flag leaf width in rice, qFLW4, which might be caused by alternative splicing of NAL1.Plant Cell Rep, 2011, 31(5): 863-872.
[22] Zhang G H, Li S Y, Wang L, et al.LSCHL4 from Japonica cultivar,which is allelic to NAL1, increases yield of Indica super rice 93-11.Mol Plant, 2014,7(8): 1350-1364.
[23] 卢扬江,郑康乐. 提取水稻DNA的一种简易方法. 中国水稻科学,1992,6(1): 47-48.
[24] Orjuela J,Garavito A,Bouniol M,et al.A universal core genetic map for rice.Theor Appl Genet, 2010,120(3): 563-572.
Outlines

/

Tel: 0571-63370278 E-mail: cjrs@263.net
Supported by Beijing Magtech Co., Ltd.