Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2015 , Vol. 29 >Issue 6: 587 - 594

DOI: https://doi.org/10.3969/j.issn.1001G7216.2015.06.004

Orginal Article

Identification and Fine Mapping of a Narrow Leaf Mutant nal10 in Rice

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  • 1College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
    State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
    Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
    College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China

*Corresponding author：E-mail:qianqian188@hotmail.com,hujiang588@163.com

Received date: 2015-01-30

  Revised date: 2015-03-17

  Online published: 2015-11-10

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Abstract

A novel rice mutant narrow leaf 10 (nal10) was identified from an EMS (ethyl methane sulphonate) mutagenized population of Wuyunjing 7. The mutant was characterized by narrow leaves and smaller architecture phenotype at the seedling stage, and dwarf, narrow leaves, deformed flowers and male sterility at the reproduction stage. Cyto-anatomical analysis indicated that the mutant had fewer large and small veins compared with the wild-type, which may account for the narrow leaf phenotype. The genetic analysis indicated the narrow leaf trait was controlled by a recessive nuclear gene. Using F2 population derived from the cross of nal10 and Taizhongbendi 1, three closely linked SSR markers RM1287, RM562 and RM5638 on chromosome 1 were obtained by the bulked segregant analysis method. With the developed new markers, the gene was finally fine-mapped to an interval of 55.2 kb between the STS markers PK83 and PK78, where a total of eight ORFs were found. RT-PCR analysis indicated the expression levels of auxin biosynthesis, signal transduction and transport related genes were all down-regulated, suggesting NAL10 is a new narrow leaf gene involving in the auxin metabolism. These results laid a foundation for further cloning of the gene and enrich the genetic regulation network of leaf development in rice.

Key words： rice; narrow leaf; NAL10; gene mapping

Cite this article

Yun-xia FANG, Li ZHU, Jiang-jie PAN, Hai-ping YU, Da-wei XUE, Yu-chun RAO, Xiao-hu WANG, Zhen-yuan SHI, Dong ZHANG, yang-zhou ZHU, Guo-jun DONG, Da-li ZENG, Guang-heng ZHANG, Long-biao GUO, Jiang HU, Qian QIAN . Identification and Fine Mapping of a Narrow Leaf Mutant nal10 in Rice[J]. Chinese Journal OF Rice Science, 2015 , 29(6) : 587 -594 . DOI: 10.3969/j.issn.1001G7216.2015.06.004

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