Chinese Journal OF Rice Science >
Development and Application of a Functional Marker for the Cold Stress Responsive Gene OsSADMC
*Corresponding author：E-mailshiyy123@163.com，wnagwensheng02@caas.cn
Received date: 2015-01-22
Revised date: 2015-03-28
Online published: 2015-09-10
Cold tolerance of 95 rice germplasms were screened at seedling stage. Among them, ten cold tolerant lines (survival rate ≥60%) and nine cold sensitive lines (survival rate=0%) were identified. Gene expression analysis of cold stress responsive gene OsSADMC showed that the expression levels of OsSADMC were significantly higher in the cold tolerant lines than those in the cold sensitive lines at both normal and cold stress conditions. Ten SNP loci were identified in the gene coding region between Lijiangxintuanheigu(LTH) and IR29. One amino acid mutation (from serine to leucine) was found between LTH and IR29. A functional marker (SADMC-CAPS1) was developed based on one SNP locus. The allele of OsSADMC carried by ten cold tolerant lines could be distinctly distinguished from that of nine cold sensitive lines by this functional marker.
Key words： rice; cold tolerance; functional marker; OsSADMC
Xiao-chen HU, Ting ZHANG, Sheng YANG, Qiao QIN, Ying-yao SHI, Wen-sheng WANG, Bin-ying FU . Development and Application of a Functional Marker for the Cold Stress Responsive Gene OsSADMC[J]. Chinese Journal OF Rice Science, 2015 , 29(5) : 475 -480 . DOI: 10.3969/j.issn.1001G7216.2015.05.004
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