Chinese Journal OF Rice Science >
Genetic Analysis and Mapping of a Dwarf and High-tillering Mutant bf370 in Rice
Received date: 2014-11-06
Revised date: 2014-12-28
Online published: 2015-07-10
A dwarf and high-tillering mutant bf370 was obtained from the indica rice variety Hong’ai B by neutron radiation. Compared with the wild type, the mutant bf370 was characterized by dwarf plant and more tillers. The number of tillers in bf370 was about 200, which was 13 times more than that in the wild type. Genetic analysis indicated that this phenotype was controlled by a single recessive nuclear gene, which was mapped to a 398 kb region between the marker Indel 4 and Indel 10 on the long arm of chromosome 1 using the F2 mapping population generated from the cross between mutant bf370 and Nipponbare. Sequencing analysis showed a 66 nucleotide deletion in the second exon of D10, resulting in a 22 amino acids deletion in the predicted RPE65 domain of D10 protein in the bf370 mutant. Combined with the phenotype of other d10 mutants, the phenotype of bf370 was likely caused by a 66 nucleotide deletion in D10.
Key words： rice; dwarf mutant; high-tillering; genetic analysis; mapping
Yun-gao HU, Guo-tao YANG, Lian-an GUO, Peng QIN, Yong-jun CHEN, Shi-gui LI . Genetic Analysis and Mapping of a Dwarf and High-tillering Mutant bf370 in Rice[J]. Chinese Journal OF Rice Science, 2015 , 29(4) : 357 -362 . DOI: 10．3969/j．issn．1001G7216．2015．04．004
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