Chinese Journal OF Rice Science >
Morphological Characteristics and Gene Mapping of a Novel Low Tiller Number and Erect Panicle Mutant in Rice (Oryza sativa L.)
Received date: 2014-11-29
Revised date: 2015-02-12
Online published: 2015-07-10
A novel rice mutant named ltep1 (low tiller number and erect panicle 1) was obtained from the wild type Nipponbare after EMS treatment. It was mainly characterized by erect panicles at the maturity stage with reduced tillers. The results showed that both rachis wall and culm wall in the ltep1 mutant were thicker than those in the wild-type plant, providing the mutant with stronger mechanical endurance. Genetic analysis indicated that the abnormal phenotype was controlled by a single recessive gene. By map-based cloning, ltep1 was mapped in a 160 kb region between two SSR markers, bep16 and RM25866, at the long arm of chromosome 10. Since no homologous gene had been found in this region, ltep1 might be a novel gene related to the development of rice panicle and tiller.
Key words： rice; erect panicle; tiller; morphological characteristics; gene mapping
Hai-bo ZHANG, Dong-dong ZENG, Xiao-li JIN, Xi ZHENG, Chun-hai SHI . Morphological Characteristics and Gene Mapping of a Novel Low Tiller Number and Erect Panicle Mutant in Rice (Oryza sativa L.)[J]. Chinese Journal OF Rice Science, 2015 , 29(4) : 350 -356 . DOI: 10．3969/j．issn．1001G7216．2015．04．003
| [1] | 袁隆平. 杂交水稻学. 北京: 中国农业出版社, 2002. |
| [2] | 陈温福, 徐正进, 张龙步. 水稻超高产育种—从理论到实践. 沈阳农业大学学报, 2003, 34(5): 324-327. |
| [3] | 徐正进, 陈温福, 张龙步. 水稻直立穗型的初步观察. 沈阳农业大学学报, 1989, 20(2): 150-153. |
| [4] | 徐正进, 陈温福, 张龙步, 等. 水稻不同穗型群体冠层光分布的比较研究. 中国农业科学, 1990, 4(1): 10-16. |
| [5] | 徐正进, 陈温福, 周洪飞, 等. 直立穗型水稻群体生理生态特性及其利用前景. 科学通报, 1996, 4(12): 1122-1126. |
| [6] | 高士杰, 张龙步, 陈温福, 等. 直立穗型水稻群体小气候环境研究. 中国农业气象, 2000, 21(3): 23-26. |
| [7] | 刘杨, 王胜强, 丁艳峰, 等. 水稻分蘖发生机理的研究进展. 中国农学通报, 2011, 27(3): 1-5. |
| [8] | Kong F N, Wang J Y, Zou J C, et al.Molecular tagging and mapping of the erect panicle gene in rice.Mol Breeding, 2007, 19(4): 297-304. |
| [9] | Yan C J, Zhou J H, Yan S, et al.Identification and characterization of a major QTL responsible for erect panicle trait in japonica rice (Oryza sativa L.).Theor Appl Genet, 2007, 115(8): 1093-1100. |
| [10] | Wang J Y, Nakazaki T, Chen S Q, et al.Identification and characterization of the erect-pose panicle gene EP conferring high grain yield in rice (Oryza sativa L.).Theor Appl Genet, 2009, 119(1): 85-91. |
| [11] | Huang X Z, Qian Q, Liu Z B, et al.Natural variation at the DEP1 locus enhances grain yield in rice.Nat Genet, 2009, 41(4): 494-497. |
| [12] | Zhou Y, Zhu J Y, Li Z Y, et al.Deletion in a quantitative trait gene qPE9-1 associated with panicle erectness improves plant architecture during rice domestication.Genetics, 2009, 183(1): 315-324. |
| [13] | Zhu K M, Tang D, Yan C J, et al.ERECT PANICLE 2 encodes a novel protein that regulates panicle erectness in indica rice.Genetics, 2010, 184(2): 343-350. |
| [14] | Li F, Liu W B, Tang J Y, et al.Rice dense and erect panicle 2 is essential for determining panicle outgrowth and elongation.Cell Res, 2010, 20(7): 838-849. |
| [15] | Piao R H, Jiang W Z, Ham T H, et al.Map-based cloning of the ERECT PANICLE 3 gene in rice.Theor Appl Genet, 2009, 119(8): 1497-1506. |
| [16] | Qiao Y L, Piao R H, Shi J X, et al.Fine mapping and candidate gene analysis of dense and erect panicle 3, DEP3, which confers high grain yield in rice (Oryza sativa L.).Theor Appl Genet, 2011, 122(7): 1439-1449. |
| [17] | Ishikawa S, Maekawa M, Arite T, et al.Suppression of tiller bud activity in tillering dwarf mutants of rice.Plant Cell Physiol, 2005, 46(1): 79-86. |
| [18] | Arite T, Iwata H, Ohshima K, et al.DWARF10, an RMS1/MAX4/DAD1 ortholog, controls lateral bud outgrowth in rice.Plant J, 2007, 51(6): 1019-1029. |
| [19] | Arite T, Umehara M, Ishikawa S, et al.d14, a strigolactone-insensitive mutant of rice, shows an accelerated outgrowth of tillers.Plant Cell Physiol, 2009, 50(8): 1416-1424. |
| [20] | Zou J, Zhang S, Zhang W, et al.The rice HIGH-TILLERING DWARF1 encoding an ortholog of Arabidopsis MAX3 is required for negative regulation of the outgrowth of axillary buds.Plant J, 2006, 48(5): 687-698. |
| [21] | Lin H, Wang R, Qian Q, et al.DWARF27, an iron-containing protein required for the biosynthesis of strigolactones, regulates rice tiller bud outgrowth.Plant Cell, 2009, 21(5):1512-1525. |
| [22] | Takeda T, Suwa Y, Suzuki M, et al.The OsTB1 gene negatively regulates lateral branching in rice.Plant J, 2003, 33(3): 513-520. |
| [23] | Li X, Qian Q, Fu Z, et al.Control of tillering in rice.Nature, 2003, 422(6932): 618-621. |
| [24] | Jiang H, Guo L B, Xue D W, et al.Genetic analysis and gene-mapping of two reduced-culm-number mutants in rice.J Integr Plant Biol, 2006, 48(3): 341-347. |
| [25] | Fujita D, Ebron L A, Araki E, et al.Fine mapping of a gene for low-tiller number, Ltn, in japonica rice (Oryza sativa L.) variety Aikawa 1.Theor Appl Genet, 2010, 120(6): 1233-1240. |
| [26] | Yasuno N, Takamure I, Kidou S, et al.Rice shoot branching requires an ATP-binding cassette subfamily G protein.New Phytol, 2009, 182(1): 91-101. |
| [27] | Michelmore R W, Paran I and Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect makers in specific genomic regions by using segregating populations.Proc Natl Acad Sci USA, 1991, 88(21): 9828-9832. |
| [28] | Shen Y, Jiang H, Jin J, et al.Development of genome-wide DNA polymorphism database for map-based cloning of rice genes.Plant Physiol, 2004, 135(3): 1198-1205. |
| [29] | 张忠旭, 陈温福, 杨振玉, 等. 水稻抗倒伏能力与茎秆物理性状的关系及其对产量的影响. 沈阳农业大学学报, 1999, 30(2): 81-85. |
| [30] | 王健, 朱锦懋, 林青青, 等. 小麦茎秆结构和细胞壁化学成分对抗压强度的影响. 科学通报, 2006, 51(6): 679-685. |
| [31] | 杨志远, 孙永健, 徐徽, 等. 不同栽培方式对免耕水稻茎鞘物质积累转运与抗倒伏能力的影响. 中国水稻科学, 2013, 27(5): 511-519. |
| [32] | 张文忠, 徐正进, 张龙步, 等. 直立穗型水稻品种演进状况分析. 沈阳农业大学学报, 2002, 33(3): 161-166. |
| [33] | Li J, Yu L, Qi G N, et al.The Os-AKT1 channel is critical for K+ uptake in rice roots and is modulated by the rice CBL1-CIPK23 complex.Plant Cell, 2014, 26(8): 3387-3402. |
| [34] | La H G, Li J, Ji Z D, et al.Genome-wide analysis of cyclin family in rice (Oryza sativa L.).Mol Gen Genomics, 2006, 275(4): 374-386. |
| [35] | Yuhko K, Shuhei Y, Hideyuki M, et al.Differential activation of the rice sucrose nonfermenting1-related protein kinase 2 family by hyperosmotic stress and abscisic acid.Plant Cell, 2004, 16(5): 1163-1177. |
/
| 〈 |
|
〉 |