Chinese Journal OF Rice Science >
Subcellular Localization Analysis of Phosphatidylserine Synthase (OsSUI1) in Tobacco Mesophyll Cells
Received date: 2014-12-23
Revised date: 2015-01-05
Online published: 2015-07-10
SUI1 encodes a phosphatidylserine synthase. And its mutant sui1-1 exhibites a shortened uppermost internode and apartly sheathed panicle. On this basis, two vectors pMDC45-SOVT and pMDC201-SOV were constructed by the Gateway system, which responds to the N-terminal and C-terminal fusion GFP. To observe the subcellular localization of SUI1, tobacco leaves have been infected by Agrobacterium with destination vectors. The results showed that GFP-SUI1 fusion proteins positioned in the plasma membrane and nucleus, while SUI1-GFP were localized in the plasma membrane and the nuclear membrane. Moreover, the distribution of the two fusion proteins were not continuous in the plasma membrane. We speculate that SUI1 protein was localized in the plasma membrane and nuclear membrane of tobacco leaf cells because the target protein folding and transmembrane manner may be changed by the N-terminal fusion, affecting the correct position of the protein.
Key words： rice; SUI1; subcellular localization
Juan ZHAO, Shu-jing KANG, Yu-chun RAO, Zhen-nan QIU, Jie XU, Jiang HU, Guang-heng ZHANG, Da-li ZENG, Long-biao GUO, Qian QIAN, Li ZHU . Subcellular Localization Analysis of Phosphatidylserine Synthase (OsSUI1) in Tobacco Mesophyll Cells[J]. Chinese Journal OF Rice Science, 2015 , 29(4) : 343 -349 . DOI: 10．3969/j．issn．1001G7216．2015．04．002
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