Differences in Al tolerance of OsPIN2 overexpressed rice seedlings (OXs) and its wild-type (WT) were investigated in 20% and 55% Al saturated soils in a pot experiment. Results indicated that in 55% Al-saturated soil, growth of root and shoot of rice seedlings was inhibited, the first tillering time was delayed, and tiller angle and numbers were reduced. OXs showed less growth inhibition both in roots and shoots than those of WT. More tiller numbers and larger tiller angles were also found in OXs than those in WT. Moreover, the contents of phosphorus, calcium and magnesium in the shoots of OXs were 1.71, 1.25 and 1.32 times of those of WT, respectively. In comparison to WT, OXs accumulated less Al in the roots, root-shoot injunction and stems, and 28.5%-109.8% more Al in the leaves. These results suggest that overexpressing of OsPIN2 in rice seedlings enhance the uptake of phosphorus, calcium and magnesium, reduce Al accumulation in roots and root-shoot injunction, and thus alleviate the toxicity of Al to rice roots and tillering under Al stress.