Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2015 , Vol. 29 >Issue 3: 223 - 231

DOI: https://doi.org/10．3969/j．issn．1001G7216．2015．03．001

ResearchPaper

OsMAX1a and OsMAX1e, Involved in the Biosynthesis of Strigolactones,Regulate Rice Tillering

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  • Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
# These authors contributed equally to this work ; * Corresponding author , E-mail: sqwangscau@163.com

Received date: 2014-11-15

  Revised date: 2014-12-23

  Online published: 2015-05-10

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Abstract

Strigolactones (SLs) are a class of phytohormone which play essential roles in regulating rice tillering, and ATMAX1 in Arabidopsis encodind P450 is an important gene involved in the synthesis of SLs.Our study found five MAX1 homologous genes through BLAST, namely OsMAX1a, OsMAX1b, OsMAX1c, OsMAX1d and OsMAX1e. Among them, OsMAX1a and OsMAX1e have stable and high expression in rice tissues. Our experiments verified that the overexpression of OsMAX1aand OsMAX1e in Arabidopsis rescued the max1 branched mutant phenotype, and the interference of OsMAX1a and OsMAX1e expression in rice increased rice tillering number. Furthermore, enogenous GR24 application inhibited the expression of OsMAX1a and OsMAX1e, and P-starvation enhanced the expression of OsMAX1a and OsMAX1e,thus promoted the biosynthesis of SLs. In addition, IAA and Kinetin(a cytokinin:KT) treatment apparently had remarkable effect on the expression of OsMAX1a and OsMAX1e. The results demonstrated that the expression of OsMAX1a and OsMAX1e responded to the SLs level and they were involved in the synthesis of SLs and regulated rice tillering.

Key words： strigolactones; OsMAX1a; OsMAX1e; tillering

Cite this article

Xiu-mei WANG, Yue-yang LIANG, Ling LI, Chang-wei GONG, Hai-peng WANG, Xiao-xi HUANG, Shuang-cheng LI, Qi-ming DENG, Jun ZHU, Ai-ping ZHENG, Ping LI, Shi-quan WANG . OsMAX1a and OsMAX1e, Involved in the Biosynthesis of Strigolactones,Regulate Rice Tillering[J]. Chinese Journal OF Rice Science, 2015 , 29(3) : 223 -231 . DOI: 10．3969/j．issn．1001G7216．2015．03．001

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