Chinese Journal OF Rice Science >
Mapping a QTL(qHTH5) for Heat Tolerance at the Heading Stage on Rice Chromosome 5 and Its Genetic Effect Analysis
Received date: 2014-10-24
Revised date: 2014-12-03
Online published: 2015-03-10
Using an interspecific near-isogenic line derived from Oryza sativa ssp. indica (Shuhui 527)×O. rufipogon Griff.(Yuanjiang common wild rice Hehuatang 3) as materials, a QTL for heat tolerance at the heading stage in the heat-tolerant near-isogenic rice line YJ10-03-01 was analyzed. A total of 360 simple sequence repeat(SSR) markers evenly distributed across 12 chromosomes were used to detect polymorphisms between YJ10-03-01 and indica restorer Shuhui 527, and nine were polymorphic. Single marker analysis revealed that markers on chromosome 5 were associated with heat tolerance at the heading stage using seed setting percentage as index for heat tolerance. By interval mapping using F2 population(1,027 plants)from YJ10-03-01×Shuhui 527, a QTL for heat tolerance was detected on the short arm of chromosome 5. The QTL explained 8.6% and 19.4% of phenotypic variances in the F2 and F3 generations under heat stress (artificial climate chamber), respectively. Recombinant plants were screened for two flanking markers, RM7320 and RM7444, in F3 generation, and substitution mapping suggested that the QTL was located in an interval of about 304.2 kb between markers RM592 and RM17921, we designated the QTL as qHTH5(quantitative trait locus for heat tolerance at the heading stage on chromosome 5).
Key words： rice(Oryza sativa L.); heat tolerance; QTL; gene mapping
Zhi-bin CAO, Hong-wei XIE, Yuan-yuan NIE, Ling-hua MAO, Yong-hui LI, Yao-hui CAI . Mapping a QTL(qHTH5) for Heat Tolerance at the Heading Stage on Rice Chromosome 5 and Its Genetic Effect Analysis[J]. Chinese Journal OF Rice Science, 2015 , 29(2) : 119 -125 . DOI: 10.3969/j.issn.1001-7216.2015.02.002
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